不同施肥模式对植烟棕壤活性有机碳组分和酶活性的影响

doi:10.7668/hbnxb.20191407

摘要/Abstract

摘要： 为探究植烟棕壤活性有机碳组分和碳转化酶活性对不同施肥模式的响应规律，以始于2009年的土壤肥力长期定位试验为研究对象，选取不施肥（CK）、单施化肥（T1）、化肥有机肥配施（T2）和单施有机肥（T3）4种田间试验处理。结果表明：较CK、T1处理，T2、T3处理显著提高了土壤总有机碳（TOC）、可溶性有机碳（DOC）、微生物量有机碳（MBC）、易氧化有机碳（ROC）、碳库管理指数（CPMI）以及纤维素酶（S-CL）、蔗糖酶（S-SC）、β-葡萄糖苷酶（S-β-GC）、多酚氧化酶（S-PPD）和过氧化物酶（S-POD）活性（P<0.05）。Pearson相关分析表明，DOC、MBC、ROC均与S-CL、S-SC、S-β-GC、S-PPD和S-POD活性之间呈极显著正相关（P<0.01）；通径分析表明，直接影响DOC、MBC和ROC含量的是S-CL、S-SC和S-β-GC，而主要起间接作用是S-PPD和S-POD。主成分分析表明，不同施肥模式对活性有机碳组分和酶活性的综合影响可分为2组：CK和T1处理为一组，T2和T3处理为一组；结合主成分分析综合得分和方差分析，不同施肥模式影响土壤活性有机碳组分和酶活性的顺序为T3 > T2 > T1 > CK。因此，单施有机肥是提升山东烟区棕壤活性有机碳组分和酶活性的最有效的施肥措施。

关键词: 植烟棕壤, 施肥模式, 活性有机碳组分, 碳转化酶活性

Abstract: To investigate the response law of organic carbon components and carbon conversion enzyme activities under different fertilization models, this study focused on long-term soil fertility tests that began in 2009, selected no fertilizer(CK), fertilization of chemical fertilizer(T1), organic manure with chemical fertilizer(T2) and fertilization of organic manure(T3) four trial processing.Compared with CK, T1 treatments, T2 and T3 treatments significantly improved TOC, DOC, MBC, ROC, CPMI and S-CL, S-SC, S-β-GC, S-PPD and S-POD.Pearson correlation analysis showed that DOC, MBC, ROC and S-CL, S-SC, S-β-GC, S-PPD and S-POD were extremely significantly positively correlated(P<0.01);Path analysis showed that S-CL, S-SC and S-β-GC directly affected the content of DOC, MBC and ROC, while S-PPD and S-POD mainly played an indirect role.Principal component analysis showed that the comprehensive effected of different fertilization models on active organic carbon components and enzyme activity could be divided into two groups:CK and T1 treatments as a group, T2 and T3 treatments as a group;combined with principal component analysis comprehensive score and analysis of variance, the order of different fertilization measured affecting soil active organic carbon components and enzyme activities was T3>T2>T1>CK. The application of organic fertilizer was the most effective fertilization measure to enhance the active organic carbon component and enzyme activity of tobacco-growing brown soil for Shandong smoke zone.

Key words: Tobacco-growing brown soil, Fertilization models, Active organic carbon components, Carbon conversion enzyme activities

中图分类号:

S158.5

王鹏, 郑学博, 梁洪波, 宋文静, 季璇, 徐艳丽, 况帅, 董建新. 不同施肥模式对植烟棕壤活性有机碳组分和酶活性的影响[J]. 华北农学报, 2021, 36(1): 187-196. doi: 10.7668/hbnxb.20191407.

WANG Peng, ZHENG Xuebo, LIANG Hongbo, SONG Wenjing, JI Xuan, XU Yanli, KUANG Shuai, DONG Jianxin. Effects of Different Fertilization Models on Active Organic Carbon Components and Enzyme Activities of Tobacco-growing Brown Soil[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 187-196. doi: 10.7668/hbnxb.20191407.

http://www.hbnxb.net/CN/Y2021/V36/I1/187

参考文献

[1] 史常亮,郭焱,朱俊峰. 中国粮食生产中化肥过量施用评价及影响因素研究[J]. 农业现代化研究,2016,37(4):671-679. doi:10.13872/j.1000-0275.2016.0064.
Shi C L,Guo Y,Zhu J F. Evaluation of over fertilization in China and its influencing factors[J]. Research of Agricultural Modernization,2016,37(4):671-679.
[2] 陈壮壮,郭俊杰,陈泽鹏,贺广生,魏彬,黄浩,谢志东,林勇,周立非,邓世媛,王维. 不同施肥模式对烤烟氮钾肥利用效率及产量和品质的影响[J]. 华北农学报,2015,30(5):180-188. doi:10.7668/hbnxb.2015.05.030.
Chen Z Z,Guo J J,Chen Z P,He G S,Wei B,Huang H,Xie Z D,Lin Y,Zhou L F,Deng S Y,Wang W.Effects of different fertilization modes on nitrogen and potassium use efficiency,yield and quality of flue-cured tobacco[J]. Acta Agriculturae Boreali-Sinaca,2015,30(5):180-188.
[3] 宋震震,李絮花,李娟,林治安,赵秉强. 有机肥和化肥长期施用对土壤活性有机氮组分及酶活性的影响[J]. 植物营养与肥料学报,2013,20(3):525-533. doi:10.11674/zwyf.2014.0302.
Song Z Z,Li X H,Li J,Lin Z A,Zhao B Q. Long-term effects of mineral versus organic fertilizers on soil labile nitrogen fractions and soil enzyme activities in agricultural soil[J]. Journal of Plant Nutrition and Fertilizers,2013,20(3):525-533.
[4] 李洪斌,张杨珠,黄运湘,胡日生,廖超林,朱列书. 不同施肥条件对烟草施肥效应及肥料利用率的影响[J]. 湖南农业科学,2013(13):48-52. doi:10.16498/j.cnki.hnnykx.2013.13.034.
Li H B,Zhang Y Z,Huang Y X,Hu R S,Liao C L,Zhu L S. Influences of different fertilizing conditions on fertilizer response and fertilizer utilization rate of tobacco[J]. Hunan Agricultural Sciences,2013(13):48-52.
[5] 肖相政,刘可星,张志红,刘亮,廖宗文. 生物有机肥对烤烟生长及相关防御性酶活性的影响[J]. 华北农学报,2010,25(1):175-179. doi:10.7668/hbnxb.2010.01.035.
Xiao X Z,Liu K X,Zhang Z H,Liu L,Liao Z W. Effect of biological organic fertilizer on flue-cured tobacco growth and defensive-related enzymes activity[J]. Acta Agriculturae Boreali-Sinica,2010,25(1):175-179.
[6] 李红丽,郭夏丽,李清飞,王岩. 抑制烟草青枯生物有机肥的研制及其生防效果研究[J]. 土壤学报,2010,47(4):798-801. doi:10.11766/trxb200804150431.
Li H L,Guo X H,Li Q F,Wang Y. Tobacco wilt suppressing bio-manure and its bio-control effect[J]. Acta Pedologica Sinica,2010,47(4):798-801.
[7] 陆海飞,郑金伟,余喜初,周惠民,郑聚峰,张旭辉,刘晓雨,程琨,李恋卿,潘根兴. 长期无机有机肥配施对红壤性水稻土微生物群落多样性及酶活性的影响[J]. 植物营养与肥料学报,2015,21(3):632-643. doi:10.11674/zwyf.2015.0310.
Lu H F,Zheng J W,Yu X C,Zhou H M,Zheng J F,Zhang X H,Liu X Y,Cheng K,Li L Q,Pan G X. Microbial community diversity and enzyme activity of red paddy soil under long-term combined inorganic-organic fertilization[J]. Journal of Plant Nutrition and Fertilizers,2015,21(3):632-643.
[8] 张长华,蒋卫,蒋玉梅,唐亮,陈方林,曹务栋,黄建国. 施肥对烤烟产量、品质及土壤养分、酶活的影响[J]. 中国土壤与肥料,2012(3):77-80. doi:10.11838/sfsc.20120316.
Zhang C H,Jiang W,Jiang Y M,Tang L,Chen F L,Cao W D,Huang J G. Influence of fertilization on the yield and quality of flue-cured tobacco,soil nutrient and enzyme activity[J]. Soil and Fertilizer Sciences in China,2012(3):77-80.
[9] 季璇,冯长春,郑学博,宋文静,陈玉蓝,况帅,董建新,杜如万. 饼肥等氮替代化肥对植烟土壤养分、酶活性和氮素利用的影响[J].中国烟草科学,2019,40(5):23-29. doi:10.13496/j.issn.1007-5119.2019.05.004.
Ji X,Feng C C,Zheng X B,Song W J,Chen Y L,Kuang S,Dong J X,Du R W. Effects of combined application of rapeseed cake and chemical fertilizers on nutrients,enzyme activity of tobacco growing soil and nitrogen utilization[J]. Chinese Tobacco Science,2019,40(5):23-29.
[10] 王雪芬,胡锋,彭新华,周虎,余喜初. 长期施肥对红壤不同有机碳库及其周转速率的影响[J]. 土壤学报,2012,49(5):954-961. doi:10.11766/trxb201106240230.
Wang X F,Hu F,Peng X H,Zhou H,Yu X C. Effects of long-term fertilization on soil organic carbon pools and their turnovers in a red soil[J]. Acta Pedologica Sinica,2012,49(5):954-961.
[11] 吴秀坤,李永梅,李朝丽,王自林,范茂攀. 纳版河流域土地利用方式对土壤总有机碳以及活性有机碳的影响[J]. 生态环境学报,2013,22(1):6-11. doi:10.3969/j.issn.1674-5906.2013.01.004.
Wu X K,Li Y M,Li C L,Wang Z L,Fan M P. Effects of land use type on soil total organic carbon and soil labile organic carbon in Naban River watershed[J]. Ecology and Environmental Sciences,2013,22(1):6-11.
[12] de Brito G S,Bautista S,López-Poma R,Pivello V R. Labile soil organic carbon loss in response to land conversion in the Brazilian woodland savanna(cerradão)[J]. Biogeochemistry,2019,144(1):31-46. doi:10.1007/s10533-019-00570-9.
[13] 倪进治,徐建民,谢正苗,王德建. 不同施肥处理下土壤水溶性有机碳含量及其组成特征的研究[J]. 土壤学报,2003,40(5):724-730. doi:10.11766/trxb200203130513.
Ni J Z,Xu J M,Xie Z M,Wang D J. Contents of WSOC and characteristics of its composition under different fertilization systems[J]. Acta Pedologica Sinica,2003,40(5):724-730.
[14] 张鹏,钟川,周泉,唐海鹰,李新梅,李萍,黄国勤. 不同冬种模式对稻田土壤碳库管理指数的影响[J]. 中国生态农业学报,2019,27(8):1163-1171. doi:10.13930/j.cnki.cjea.180995.
Zhang P,Zhong C,Zhou Q,Tang H Y,Li X M,Li P,Huang G Q. Effects of different winter planting patterns on carbon management index of paddy field[J]. Chinese Journal of Eco-Agriculture,2019,27(8):1163-1171.
[15] Tang H M,Li C,Xiao X P,Pan X C,Cheng K K,Shi L H,Li W Y,Wen L,Wang K. Effects of long-term fertilizer regime on soil organic carbon and its labile fractions under double cropping rice system of southern China[J]. Acta Agriculture Scandinavica,Section B-Soil & Plant Science,2020,70(5):409-418.doi:10.1080/09064710.2020.1758763.
[16] Li S,Zhang S R,Pu Y L,Li T,Xiao X X,Jia Y X,Deng O P,Guo S G. Dynamics of soil labile organic carbon fractions and C-cycle enzyme activities under straw mulch in Chengdu plain[J]. Soil and Tillage Research,2016,155:289-297. doi:10.1016/j.still.2015.07.019.
[17] Sinsabaugh R L. Phenol oxidase,peroxidase and organic matter dynamics of soil[J]. Soil Biology and Biochemistry,2010,42(3):391-404. doi:10.1016/j.soilbio.2009.10.014.
[18] 李月,张水清,韩燕来,李世莹,王祎,李慧,李芳,谭金芳. 长期秸秆还田与施肥对潮土酶活性和真菌群落的影响[J]. 生态环境学报,2020,29(7):1359-1366. doi:10.16258/j.cnki.1674-5906.2020.07.010.
Li Y,Zhang S Q,Han Y L,Li S Y,Wang Y,Li H,Li F,Tan J F. Effects on fluvo-aquic soil enzyme activity and fungal community of long-term straw returning and fertilization[J]. Ecology and Environmental Sciences,2020,29(7):1359-1366.
[19] 马晓霞,王莲莲,黎青慧,李花,张树兰,孙本华,杨学云. 长期施肥对玉米生育期土壤微生物量碳氮及酶活性的影响[J]. 生态学报,2012,32(17):5502-5511. doi:10.5846/stxb201110191555.
Ma X X,Wang L L,Li Q H,Li H,Zhang S L,Sun B H,Yang X Y. Effects of long-term fertilization on soil microbial biomass carbon and nitrogen and enzyme activities during maize growing season[J]. Acta Ecologica Sinica,2012,32(17):5502-5511.
[20] Zhang Y P,Liu Z H,Li Y,Zhong Z W,Sun M,Jing Y P. Effects of long-term located fertilization on wheat yield and soil nutrients of three types of soils in Shandong Province[J]. Agricultural Science & Technology,2014,15(3):400-406. doi:10.16175/j.cnki.1009-4229.2014.03.027.
[21] 闫慧峰,梁洪波,许家来,李德成,杨举田,杜传印,牛柱峰,刘光亮,徐宜民,肖振杰,关辉,石屹. 山东烟叶生产典型样区土壤质量评价[J]. 中国土壤与肥料,2015,6(7):41-47. doi:10.11838/sfsc.20150607.
Yan H F,Liang H B,Xu J L,Li D C,Yang J T,Du C Y,Niu Z F,Liu G L,Xu Y M,Xiao Z J,Guan H,Shi Y. Evaluation of soil quality in Shandong typical tobacco production region[J]. Soil and Fertilizer Sciences in China,2015,6(7):41-47.
[22] 周伟,吴红慧,张运龙,徐明岗,Aslam M,文石林. 土壤活性有机碳测定方法的改良[J]. 土壤通报,2019,50(1):70-75. doi:10.19336/j.cnki.trtb.2019.01.11.
Zhou W,Wu H H,Zhang Y L,Xu M G,Aslam M,Wen S L. Improvement of determination method for soil labile organic carbon[J]. Chinese Journal of Soil Science,2019,50(1):70-75.
[23] Vance E D,Brookes P C,Jenkinson D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology and Biochemistry,1987,19(6):703-707. doi:10.1016/0038-0717(87) 90052-6.
[24] 陈安强,付斌,鲁耀,段宗颜,胡万里. 有机物料输入稻田提高土壤微生物碳氮及可溶性有机碳氮[J]. 农业工程学报,2015,31(21):160-167.doi:10.11975/j.issn.1002-6819.2015.21.021.
Chen A Q,Fu B,Lu Y,Duan Z Y,Hu W L. Exogenous organic materials applied to paddy field improving soil microbial biomass C,N and dissolved organic C,N[J]. Transactions of the Chinese Society of Agricultural Engineering,2015,31(21):160-167.
[25] 马维伟,李广,宋捷,闫丽娟,武利玉. 植被退化对尕海湿地土壤有机碳库及碳库管理指数的影响[J]. 草地学报,2019,27(3):687-694. doi:10.11733/j.issn.1007-0435.2019.03.022.
Ma W W,Li G,Song J,Yan L J,Wu L Y. Effect of vegetation degradation on soil organic carbon pool and carbon pool management index in the Gahai Wetland,China[J]. Acta Agrestia Sinica,2019,27(3):687-694.
[26] 董扬红,曾全超,安韶山,张宏. 黄土高原不同林型植被对土壤活性有机碳及腐殖质的影响[J]. 水土保持学报,2015,29(1):143-148. doi:10.13870/j.cnki.stbcxb.2015.01.029.
Dong Y H,Zeng Q C,An S S,Zhang H. Effects of different forest types on soil active organic carbon and soil humus composition in the Loess Plateau[J]. Journal of Soil and Water Conservation,2015,29(1):143-148.
[27] 滕臻,曹小青,孙孟瑶,李培玺,徐小牛. 不同生态恢复模式对巢湖湖滨湿地土壤活性碳库及其管理指数的影响[J]. 生态环境学报,2019,28(4):752-760. doi:10.16258/j.cnki.1674-5906.2019.04.014.
Teng Z,Cao X Q,Sun M Y,Li P X,Xu X N. Effect of different ecological restoration patterns on soil labile organic carbon and carbon pool management index of lakeside wetland of Chaohu Lake[J]. Ecology and Environmental Sciences,2019,28(4):752-760.
[28] García-Ruiz R,Ochoa V,Hinojosa M B,Carreira J A. Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems[J]. Soil Biology and Biochemistry,2008,40(9):2137-2145. doi:10.1016/j.soilbio.2008.03.023.
[29] 张玉军,黄绍敏,李斌,龙潜,姜桂英,申凤敏,刘世亮. 长期施肥对潮土不同层次活性有机质及碳库管理指数的影响[J]. 水土保持学报,2019,33(3):160-165. doi:10.13870/j.cnki.stbcxb.2019.03.024.
Zhang Y J,Huang S M,Li B,Long Q,Jiang G Y,Shen F M,Liu S L. Effects of long-term fertilization on the labile organic carbon and carbon pool management index at different layers in fluvo-aquic soil[J]. Journal of Soil and Water Conservation,2019,33(3):160-165.
[30] 皇甫呈惠,孙筱璐,刘树堂,贾志越,赵洪翠. 长期定位秸秆还田对土壤团聚体及有机碳组分的影响[J]. 华北农学报,2020,35(3):153-159. doi:10.7668/hbnxb.20190844.
Huangfu C H,Sun X L,Liu S T,Jia Y Z,Zhao H C. Effects of long-term straw returning to field on soil aggregates and organic carbon components[J]. Acta Agriculturae Boreali-Sinica,2020,35(3):153-159.
[31] 张迪,韩晓增,侯雪莹. 长期不同施肥管理对黑土活性有机碳及碳库管理指数的影响[J]. 土壤通报,2011,42(3):654-658. doi:10.19336/j.cnki.trtb.2011.03.028.
Zhang D,Han X C,Hou X Y. The effects of long-term fertilization on the labile organic carbon and carbon management index in black soil[J]. Chinese Journal of Soil Science,2011,42(3):654-658.
[32] Ge T D,Wu X H,Chen X J,Yuan H Z,Zou Z Y,Li B Z,Zhou P,Liu S L,Tong C L,Brookes P,Wu J S. Microbial phototrophic fixation of atmospheric CO₂ in China subtropical upland and paddy soils[J]. Geochimica et Cosmochimica Acta,2013,113:70-78. doi:10.1016/j.gca.2013.03.020.
[33] 丁少男,薛萐,刘国彬. 施肥处理对黄土丘陵区农田土壤酶活性和水溶性有机碳、氮的影响[J]. 农业环境科学学报,2015,34(11):2146-2154. doi:10.11654/jaes.2015.11.016.
Ding S N,Xue S,Liu G B. Effects of fertilization on soil enzyme activities and water-soluble organic carbon and nitrogen contentin farmland on Hilly Loess Plateau[J]. Journal of Agro-Environment Science,2015,34(11):2146-2154.
[34] Zhang L G,Chen X,Xu Y J,Jin M C,Ye X X,Gao H J,Chu W Y,Mao J D,Thompson M L. Soil labile organic carbon fractions and soil enzyme activities after 10 years of continuous fertilization and wheat residue incorporation[J]. Scientific Reports,2020,10(1):80-99.doi:10.1038/s41598-020-68163-3.
[35] 李艳鹏,贺同鑫,王清奎. 施肥对杉木林土壤酶和活性有机碳的影响[J]. 生态学杂志,2016,35(10):2722-2731. doi:10.13292/j.1000-4890.201610.028.
Li Y P,He T X,Wang Q K. Impact of fertilization on soil organic carbon and enzyme activities in a Cunninghamia lanceolata plantation[J]. Chinese Journal of Ecology,2016,35(10):2722-2731.
[36] 刘兰清,杨晨璐,王维钰,孔德杰,Akhtar K,任广鑫,冯永忠,杨改河. 免耕条件下秸秆还田与施肥对小麦-玉米轮作系统土壤养分和酶活性的影响[J]. 华北农学报,2017,32(6):213-221.doi:10.7668/hbnxb.2017.06.031.
Liu L Q,Yang C L,Wang W Y,Kong D J,Akhtar K,Ren G X,Feng Y Z,Yang G H. Effects of straw returning to field and fertilization on soil nutrient and enzyme activity in wheat-maize rotation system under no-tillage condition[J]. Acta Agriculturae Boreali-Sinica,2017,32(6):213-221.
[37] Borase D N,Nath C P,Hazra K K,Senthilkumar M,Singh S S,Praharaj C S,Singh U,Kumar N. Long-term impact of diversified crop rotations and nutrient management practices on soil microbial functions and soil enzymes activity[J]. Ecological Indicators,2020,114:1-11. doi:10.1016/j.ecolind.2020.106322.
[38] 田小明,李俊华,危常州,褚贵新. 不同生物有机肥用量对土壤活性有机质和酶活性的影响[J]. 中国土壤与肥料,2012(1):26-32. doi:10.11838/sfsc.20120105.
Tian X M,Li J H,Wei C Z,Chu G X. Effect of different bio-organic fertilizer application rate on soil active organic matter and enzyme activities[J]. Soil and Fertilizer Sciences in China,2012(1):26-32.
[39] 李增强,张贤,王建红,曹凯,徐昌旭,曹卫东. 紫云英施用量对土壤活性有机碳和碳转化酶活性的影响[J]. 中国土壤与肥料,2018(4):14-20. doi:10.11838/sfsc.20180403.
Li Z Q,Zhang X,Wang J H,Cao K,Xu C X,Cao W D. Effects of Chinese milk vetch(Astragalus sinicus L.) application rate on soil labile organic carbon and C-transformation enzyme activities[J]. Soil and Fertilizer Sciences in China,2018(4):14-20.
[40] Grandy A S,Neff J C,Weintraub M N. Carbon structure and enzyme activities in alpine and forest ecosystems[J]. Soil Biology and Biochemistry,2007,39(11):2701-2711. doi:10.1016/j.soilbio.2007.05.009.
[41] 魏俊杰,洪坚平. 无机有机肥配施生物炭对复垦土壤酶活性以及磷形态的影响[J]. 华北农学报,2019,34(6):170-176. doi:10.7668/hbnxb.20190240.
Wei J J,Hong J P. Effects of inorganic organic fertilizers combined with biochar on enzyme activities and phosphorus forms in reclaimed soil[J]. Acta Agriculturae Boreali-Sinica,2017,32(6):170-176.
[42] Straten P T,Guldberg P,Schrama D,Andersen M H,Moerch U,Seremet T,Siedel C,Reisfeld R A,Becker J C. In situ cytokine therapy:Redistribution of clonally expanded T cells[J]. European Journal of Immunology,2001,31(1):250-258.doi:10.1002/1521-4141(200101) 31:1<250∷AID-IMMU250>3.0.CO;2-8.
[43] Holík L,Hlisnikovský L,Honzík R,Trögl J,Burdová H,Popelka J. Soil microbial communities and enzyme activities after long-term application of inorganic and organic fertilizers at different depths of the soil profile[J]. Sustainability,2019,11(12):3251.doi:10.3390/su11123251.
[44] 柳开楼,韩天富,胡惠文,黄庆海,余喜初,李大明,叶会财,胡志华.红壤旱地玉米开花期土壤酶活性对长期施肥的响应[J]. 植物营养与肥料学报,2018,24(6):1610-1618. doi:10.11674/zwyf.18155.
Liu K L,Han T F,Hu H W,Huang Q H,Yu X C,Li D M,Ye H C,Hu Z H. Response of soil enzyme activity in flowering stages of maize to long-term fertilization in red soil[J]. Journal of Plant Nutrition and Fertilizers,2018,24(6):1610-1618.
[45] Yu H Y, Ding W X, Luo J F, Donnisona A,Zhang J B. Long-term effect of compost and inorganic fertilizer on activities of carbon-cycle enzymes in aggregates of an intensively cultivated sandy loam[J]. Soil Use and Management,2012,28(3):347-360.doi:10.1111/j.1475-2743.2012.00415.x.
[46] 李增强,张贤,王建红,曹凯,徐昌旭,曹卫东.化肥减施对紫云英还田土壤活性有机碳和碳转化酶活性的影响[J]. 植物营养与肥料学报,2019,25(4):525-534. doi:10.11674/zwyf.18121.
Li Z Q,Zhang X,Wang J H,Cao K,Xu C X,Cao W D. Effect of chemical fertilizer reduction with return of Chinese milk Vetch(Astragalus sinicus L.) on soil labile organic carbon and carbon conversion enzyme activities[J]. Journal of Plant Nutrition and Fertilizers,2019,25(4):525-534.

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