盐胁迫与非胁迫条件下控制番茄苗期重要植物学性状的QTL对比研究

doi:10.7668/hbnxb.2011.05.013

华北农学报 ›› 2011, Vol. 26 ›› Issue (5): 58-65. doi: 10.7668/hbnxb.2011.05.013

所属专题： 番茄；盐碱胁迫；

盐胁迫与非胁迫条件下控制番茄苗期重要植物学性状的QTL对比研究

黄亚杰, 杜永臣, 侯喜林, 王孝宣, 高建昌, 国艳梅

1. 中国农业科学院蔬菜花卉研究所, 北京 100081;
2. 南京农业大学园艺学院, 江苏南京210095

收稿日期:2011-05-12 出版日期:2011-10-28
通讯作者: 杜永臣( 1955-) ，男，河北昌黎人，研究员，博士生导师，主要从事番茄遗传育种研究。
作者简介:黄亚杰( 1985-) ，女，河北沧州人，硕士研究生，主要从事番茄遗传育种研究。
基金资助:
国家自然科学基金(30771474;30900988);国家“863”项目(2007AA10Z178);北京市自然科学基金项目(6102020)

Comparative QTL Analysis for Phytological Traits of Tomato during Vagetative Stage under Salt Stress and Nonstress Conditions

HUANG Ya-jie, DU Yong-chen, HOU Xi-lin, WANG Xiao-xuan, GAO Jian-chang, GUO Yan-mei

1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Received:2011-05-12 Published:2011-10-28

摘要/Abstract

摘要： 利用来源于栽培番茄9706和野生契斯曼尼番茄LA0317杂交后的130个BC2S4株系为群体,采用营养液栽培法,在盐胁迫与非胁迫环境下分别对株高、茎粗、根鲜质量、根干质量、地上鲜质量、地上干质量以及耐盐级数进行了QTL分析。盐胁迫环境中检出13个QTL:株高2个;茎粗2个;根鲜质量2个;根干质量2个;地上鲜质量3个;地上干质量2个。非盐胁迫环境中检出32个QTL:株高6个;茎粗2个;根鲜质量6个;根干质量6个;地上鲜质量6个;地上干质量6个。另外,在盐胁迫条件下利用耐盐级数定位到2个耐盐性QTL,分别位于第3,6染色体上。通过盐胁迫与非胁迫下的QTL对比研究发现,第2,3,8染色体上的QTL仅在盐胁迫条件下能检测到,第9,12染色体上的QTL仅在非盐胁迫条件下能检测到,不同性状间的QTL存在共定位现象。同时,研究获得了5个表型和QTL分析均极耐盐的育种材料,可用于番茄耐盐新品种选育。

关键词: 番茄, 盐胁迫, 非盐胁迫, QTL分析

Abstract: An advanced backcross population of 130 BC2S4 individuals was developed derived from the cross Solanum lycopersicum 9706 × Solanum cheesmanii LA0317.Six important phytological traits were investigated under salt stress and nonstress conditions,and salt survival value was also evaluated.Using MQM mapping method,a total of 45 QTL for phytological traits of tomato were detected.2 QTL for salt tolerance were mapped to chromosome 3 and 6.Through comparative QTL analysis,it was found that QTL on chromosome 2,3 and 8 could only be detected under salt treatment,while QTL on chromosome 9 and 12 could only be detected under non-salt condition.QTL co-location among different traits was common in this study.Moreover,5 families of BC2S4 were found in this paper to be salt tolerant,thus these lines could be used as good breeding germplasm.

Key words: Tomato, Salt stress, Nonstress, QTL mapping

中图分类号:

S641.2

黄亚杰, 杜永臣, 侯喜林, 王孝宣, 高建昌, 国艳梅. 盐胁迫与非胁迫条件下控制番茄苗期重要植物学性状的QTL对比研究[J]. 华北农学报, 2011, 26(5): 58-65. doi: 10.7668/hbnxb.2011.05.013.

HUANG Ya-jie, DU Yong-chen, HOU Xi-lin, WANG Xiao-xuan, GAO Jian-chang, GUO Yan-mei. Comparative QTL Analysis for Phytological Traits of Tomato during Vagetative Stage under Salt Stress and Nonstress Conditions[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 58-65. doi: 10.7668/hbnxb.2011.05.013.

http://www.hbnxb.net/CN/Y2011/V26/I5/58

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盐胁迫与非胁迫条件下控制番茄苗期重要植物学性状的QTL对比研究

Comparative QTL Analysis for Phytological Traits of Tomato during Vagetative Stage under Salt Stress and Nonstress Conditions

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盐胁迫与非胁迫条件下控制番茄苗期重要植物学性状的QTL对比研究

Comparative QTL Analysis for Phytological Traits of Tomato during Vagetative Stage under Salt Stress and Nonstress Conditions

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