双分子荧光互补试验研究水稻NH1家族蛋白之间相互作用

doi:10.7668/hbnxb.2011.02.007

华北农学报 ›› 2011, Vol. 26 ›› Issue (2): 30-34. doi: 10.7668/hbnxb.2011.02.007

所属专题： 水稻；

双分子荧光互补试验研究水稻NH1家族蛋白之间相互作用

白薇¹, 孙海莲², MawshengChern³, RandyRuan³, PamRonald³, 樊明寿⁴

1. 内蒙古农业大学生命科学学院, 内蒙古呼和浩特, 010018;
2. 中国科学院内蒙古草业研究中心, 内蒙古呼和浩特, 010031;
3. DepartmentofPlantPathology, UniversityofCalifornia, Davis, CA, 95616. USA;
4. 内蒙古农业大学农学院, 内蒙古呼和浩特, 010018

收稿日期:2010-12-12 出版日期:2011-04-28
作者简介:白薇(1972-),女,内蒙古包头人,副教授,博士,主要从事植物抗病分子研究.
基金资助:
美国农业部USDANRIgrant(#2004-63560416640)

Protein-Protein Interaction Between Members of NH1 Family in Bimolecular Fluorescence Complementation Assay

BAI Wei¹, SUN Hai-lian², Mawsheng Chern³, Randy Ruan³, Pam Ronald³, FAN Ming-shou⁴

1. College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China;
2. Inner Mongolia Grassland Research Center, Chinese Academy of Sciences, Huhhot 010031, China;
3. Department of Plant Pathology, University of California, Davis, CA 95616, USA;
4. College of Agronomy, Inner Mongolia Agricultural University, Huhhot 010018, China

Received:2010-12-12 Published:2011-04-28

摘要/Abstract

摘要： NPR1(Non-expresser of pathogenesis related genes 1)是水杨酸(Salicilic acid,SA)介导的系统获得抗性(Systemic aquired resistance,SAR)的关键调控因子,在水稻中过量表达拟南芥NPR1和水稻NH1⁄OsNPR1(NPR1 homo1ogue 1)(NPR1的同源物)可增强抗病性.水稻基因组中还有4个NPR1旁系同源物(Paralog),为NH1的家族蛋白.本试验利用双分子荧光互补技术(Bimolecular Fluorescence Complementation,BiFC,or split YFP)研究了水稻NH1家族蛋白之间在活体内的相互作用,发现除NH2外,NH1、NH3、NH4和NH都会和自身的蛋白互作,产生荧光信号,并且它们还会和家族成员的其他蛋白相互结合产生荧光信号.

关键词: 水稻, NH1家族蛋白, 双分子荧光互补试验, 蛋白互作

Abstract: NPRl(Non-expresser of pathogenesis related genes 1)is the master regulator of salicylic acid-mediated systemic acquired resistance.Over-expression of Arabidopsis NPRI and rice NHl(NPRl homologl)/OsNPRl in rice resuhs in enhanced resistance.There are four rice NPRl-paralogs in the rice genome,they are family members of NHl.Protein-protein interaction between members of NHl family were studied in vivo by bimolecular fluorescence eomplementation assay(BiFC,Or split YFP).NHl,NH3,NH4,and NH5 all can interact with itself to generate fluores-eence signals.They Can also interact with other members in the family.But NH2 has no interaction with anyone.

Key words: Rice, NH1 family members Bimolecular fluorescence complementation assay, Protein-protein interaction

中图分类号:

白薇, 孙海莲, MawshengChern, RandyRuan, PamRonald, 樊明寿. 双分子荧光互补试验研究水稻NH1家族蛋白之间相互作用[J]. 华北农学报, 2011, 26(2): 30-34. doi: 10.7668/hbnxb.2011.02.007.

BAI Wei, SUN Hai-lian, Mawsheng Chern, Randy Ruan, Pam Ronald, FAN Ming-shou. Protein-Protein Interaction Between Members of NH1 Family in Bimolecular Fluorescence Complementation Assay[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 30-34. doi: 10.7668/hbnxb.2011.02.007.

http://www.hbnxb.net/CN/Y2011/V26/I2/30

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双分子荧光互补试验研究水稻NH1家族蛋白之间相互作用

Protein-Protein Interaction Between Members of NH1 Family in Bimolecular Fluorescence Complementation Assay

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双分子荧光互补试验研究水稻NH1家族蛋白之间相互作用

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