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玉米弯孢菌叶斑病菌致病性分化的RAPD鉴定

鄢洪海   

  1. 青岛农业大学 植物保护学院, 山东青岛 266109
  • 收稿日期:2009-01-20 出版日期:2009-12-31
  • 作者简介:鄢洪海(1964 -), 男, 山东德州人, 副教授, 博士, 主要从事植物病理生理学教学和科研工作。
  • 基金资助:
    国家“九五”科技攻关项目(96005010303);青岛市自然科学基金项目(05-JC-55)

Study on RAPD for Physiological Differentiation of Curvularia lunata in Maize

YANHong-hai   

  1. College of Plant Protection Qingdao Agricultural University, Qingdao 266109, China
  • Received:2009-01-20 Published:2009-12-31

摘要: 从60条随机引物中选择12对组合,对玉米弯孢叶斑病菌基因组DNA进行随机扩增,共扩增出128条遗传标记。根据这些标记使用Statistic统计软件对菌株之间相互关系进行聚类分析,结果表明:菌株间最高相似系数是为87%,最低为47%。22个供试菌株在连锁距离0.4范围内全部聚在一起,树状图上的菌株可分成5~6组。a组包括:98-36、98-25、98-17、98-07、98-01和98-28 6个菌株,是遗传基础最接近的一组。

关键词: 玉米弯孢叶斑病菌, 致病性分化, DNA, 多态性

Abstract: One hundred and twenty -eight piece of belt signs are multiplied through selecting 12 pairs of associationsform 60 piece of random decoy and multiplying Curvularia lunata randomly.According to the signs,pathogen's mutual relationsare analyzed.The result is that the highest similarity degree of the pathogen is 87 % and the lowest is 47 %.Twenty-two experimental pathogen all gather together by all stems on 0.4 to the stem in the linked together.The pathogenforming tree shape can be divided into 5 or 6 groups.Group a consists of 6 pathogen,they are 98 -36,98 -25,98 -17 ,98 -07,98 -01 and 98 -28 ,which is the most approximate one on heredity base.

Key words: Curvularalunata, Pathogenicitydifferentiation, DNA, Muchappearancesofheredity

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引用本文

YANHong-hai. Study on RAPD for Physiological Differentiation of Curvularia lunata in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.060.

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链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.2009.S1.060

               http://www.hbnxb.net/CN/Y2009/V24/IS1/258