摘要： 近年来在北方由于蔬菜大棚的大力发展,为根结线虫的越冬提供了良好的生活环境,保护地蔬菜根结线虫的发生日趋严重,为了鉴定危害山东省保护地蔬菜根结线虫的种类,从山东蔬菜主产区7个地市的蔬菜生产大棚中采集了14种蔬菜根结线虫寄主样本,采用ITS-PCR分子生物学鉴定方法对其进行了鉴定,表明山东蔬菜主产区的蔬菜根结线虫均为南方根结线虫(Meloidogyine incognita)。

关键词: 蔬菜, 南方根结线虫, ITS序列

Abstract: Root -knot nematodes(Meloidogyne spp.)are important endoparasitic pests of numerous crop species.Most vegetables are highly susceptible host of several species of root -knot nematodes and incurs yield losses from root -knot infection.The yield of vegetables in greenhouse is increaseing,but nematodes in greenhouse and other controlled environmentproduction systems propagate rapidly.To ascertain the species of the root -knot nematodes ,fourteen samples of root-knot nematodewere collected in the greenhouse planted with various vegetables from seven city of Shandong Province.Thesequences of ITS region of nematode were amplified by PCR.The LTS sequence of nematode collected was compared withthe data of NCBI by BLAST.The results showed that all samples of root -knot nematodes belong to Meloidogyine incognita.

Key words: Vegetable, Meloidogyineincognita, ITSsequence

中图分类号: 

• S436.3