华北农学报 ›› 2009, Vol. 24 ›› Issue (6): 69-73. doi: 10.7668/hbnxb.2009.06.014

所属专题: 小麦 生物技术

• 论文 • 上一篇    下一篇

铬胁迫下三种基因型小麦萌发和抗氧化酶差异的研究

张黛静1, 姜丽娜2, 邵云1, 李召虎3, 李春喜2   

  1. 1. 中国农业大学, 农学与生物技术学院,北京 100193;
    2. 河南师范大学, 生命科学学院,河南 新乡 453007;
    3. 中国农业大学, 农学与生物技术学院,北京 100193
  • 收稿日期:2009-10-08 出版日期:2009-12-28
  • 通讯作者: 李春喜(1964-),男,河南封丘人,教授,主要从事小麦栽培生理研究。
  • 作者简介:张黛静(1974-),女,河南新乡人,在读博士,主要从事小麦逆境生理研究.
  • 基金资助:
    "十一五"国家科技支撑计划(2006BAD02A15;2006BAK02A25)

Differential Responses in Germination and Antionxidant Enzymes of Three Wheat Cultivars to Chromium Stress

ZHANG Dai-jing1, JIANG Li-na2, SHAO Yun1, LI Zhao-hu3, LI Chun-xi2   

  1. 1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;
    2. College of Life Sciences, Henan Normal University, Xinxiang 453007, China
  • Received:2009-10-08 Published:2009-12-28

摘要: 采用3种不同基因型小麦品种(新麦19、矮抗8和豫麦18),对其进行萌发期不同浓度Cr2(SO4)3胁迫处理.研究幼苗的发芽势、发芽率、根长、芽长、叶绿素、根系活力、MDA含量,以及抗氧化酶的变化.结果表明:随Cr浓度的增加,发芽势、发芽率和芽长均基本呈"先升后降"趋势;同一浓度Cr处理下,发芽率、发芽势和芽长均表现为新麦19>矮抗8>豫麦18,根长为豫麦18>新麦19>矮抗8;10 mg/L的Cr胁迫下,小麦幼芽叶绿素含量、幼根根系活力降低,MDA含量升高;随Cr浓度的升高,幼芽SOD、POD活性升高;SOD、POD活性在新麦19中变幅最小.

关键词: 小麦, 铬, 萌发, 生理活性, 抗氧化酶

Abstract: Chromium(Cr)effects the growth of wheat plants(Triticum aestivum L.)badly.In this experiment,the effects of Cr toxicityon Chloraphyll content,root activity,malondialdehyde(MDA) content and antioxidative eniymes activity in seedlings were investigated to clarify different Cr resistibility in three wheat cultivars.The results showed:with the increase of Cr concentration,the germination potential,germination percentage,root length and shoot length increased first then declined.Under the same Cr concentration treatment,the characteristics of germination energy,germination percentage and shoot length presented Xinmai 19 >Aikang 58 > Yumai 18 the characteristics of root length presented Yumai 18 > Xinmai 19 >Aikang 581150 mg/L chromium treatment,chlorophyll content,root activities inhibitied heavy exposed to excess Cr.MDA content,SOD and POD content increased significantly in wheat shoots exposed to excess Cr.The SOD and POD activity also differed for the three cultivars.Cr stress significant promoted SOD and POD content in Yumai18 than Xinmai 19 and Aikang 58.

Key words: Wheat, Chromium, Germination, Physiological activities, Antionxidant enzymes

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引用本文

张黛静, 姜丽娜, 邵云, 李召虎, 李春喜. 铬胁迫下三种基因型小麦萌发和抗氧化酶差异的研究[J]. 华北农学报, 2009, 24(6): 69-73. doi: 10.7668/hbnxb.2009.06.014.

ZHANG Dai-jing, JIANG Li-na, SHAO Yun, LI Zhao-hu, LI Chun-xi. Differential Responses in Germination and Antionxidant Enzymes of Three Wheat Cultivars to Chromium Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 69-73. doi: 10.7668/hbnxb.2009.06.014.

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