华北农学报 ›› 2008, Vol. 23 ›› Issue (S2): 92-95. doi: 10.7668/hbnxb.2008.S2.020

所属专题: 小麦 生物技术

• 论文 • 上一篇    下一篇

60Coγ射线与EMS对小麦近等基因系TcLr10的复合诱变

程志锋, 杨文香, 刘大群   

  1. 河北农业大学, 植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 河北, 保定, 071000
  • 收稿日期:2008-08-14 出版日期:2008-12-31
  • 通讯作者: 刘大群( 1958- ), 男, 河北石家庄人, 博士生导师, 主要从事植物病害生物防治和分子植物病理学研究。
  • 作者简介:程志锋(1982-),男,河北峰峰矿区人,硕士,主要从事分子植物病理学研究.
  • 基金资助:
    重大基础研究前期专项(2005CCA01600)

Compound Mutagenesis on Near-isogenic Lines TcLr10 by 60Co γRay and EMS in Wheat

CHENG Zhi-feng, YANG Wen-xiang, LIU Da-qun   

  1. Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China
  • Received:2008-08-14 Published:2008-12-31

摘要: 为获得60Coγ射线与EMS对小麦近等基因系TcLr10的复合诱变体系,研究以小麦抗叶锈近等基因系为材料,应用60Coγ射线进行辐照并用EMS溶液进行处理,对小麦的发芽率、三叶期、致死率及抗病性进行分析。结果表明,本试验所用的诱变剂量对小麦近等基因的发芽率影响不显著。所用处理中到达三叶期的时间基本相同,但三叶期到四叶期的时间却随着浓度的增加而减少。当辐照剂量达到或大于0.15 kGy时,致死率显著增加。在EMS浓度为0.4%,60Coγ射线辐照剂量为0.15 kGy时,得到了3株小麦抗叶锈病近等基因系的突变体所得最佳诱变组合为EMS浓度为0.4%,γ射线辐照剂量0.15 kGy。

关键词: 小麦叶锈病, 近等基因系, 复合诱变, EMS

Abstract: 60Co γ ray and EMS were used to treat wheat leaf rust resistance gene lineTcLr10 in order to establish the compound mutagenesis system for wheat near iso??genetic line.The germination rate, trefoil stage, fatal rate and disease re??sistance after treatment were investigated.The result showed that the effect of germination rate was not significant affectedby the treatment of60Co γ ray and EMS at different dosage.The wheat was nearly reach trefoil stage at the same time inevery mutagenesis dose.However, the time required from trefoil stage to quatrefoil stage was decreased with the concentra??tion increase.The fatal rate remarkably increased when the irradiationdose was or over 0. 15 kGy. Three mutants of near??isogenic lines TcLr10 in wheat were obtained when the concentration of EMS was 0. 4% and the irradiation dose of 60Co ray was 0.15 kGy.The effect of compound mutagenesis on Tclr10 was the best when the irradiation dose was 0. 15 kGyand the concentration of EMS was 0. 4%.

Key words: Wheat leaf rust, Near??isogenic lines, Compound mutagenesis, EMS

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引用本文

程志锋, 杨文香, 刘大群. 60Coγ射线与EMS对小麦近等基因系TcLr10的复合诱变[J]. 华北农学报, 2008, 23(S2): 92-95. doi: 10.7668/hbnxb.2008.S2.020.

CHENG Zhi-feng, YANG Wen-xiang, LIU Da-qun. Compound Mutagenesis on Near-isogenic Lines TcLr10 by 60Co γRay and EMS in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 92-95. doi: 10.7668/hbnxb.2008.S2.020.

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