小麦河农6251苗期抗叶锈病基因的鉴定

doi:10.7668/hbnxb.2015.03.033

华北农学报 ›› 2015, Vol. 30 ›› Issue (3): 192-199. doi: 10.7668/hbnxb.2015.03.033

所属专题： 小麦；生物技术；

小麦河农6251苗期抗叶锈病基因的鉴定

任晓娣¹, 杜冬冬¹, 张河山¹, 姚宏鹏¹, 赵赛¹, 杨文香^1,2, 刘大群^1,2,3

1. 河北农业大学植物病理学系, 河北保定 071001;
2. 河北省农作物病虫害生物防治工程技术研究中心, 河北保定 071001;
3. 国家北方山区农业工程技术研究中心, 河北保定 071001

收稿日期:2015-02-20 出版日期:2015-06-28
通讯作者: 杨文香(1966-),女,河北沧州人,教授,博士,博士生导师,主要从事分子植物病理学及植物病害生物防治研究;刘大群(1958-),男,河北石家庄人,教授,博士,博士生导师,主要从事分子植物病理学及植物病害生物防治研究。
作者简介:任晓娣(1987-),女,河北邯郸人,硕士,主要从事分子植物病理学研究。
基金资助:
国家重点基础研究发展计划项目(2013CB127700);河北省现代农业产业体系小麦产业创新团队建设项目

Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage

REN Xiao-di¹, DU Dong-dong¹, ZHANG He-shan¹, YAO Hong-peng¹, ZHAO Sai¹, YANG Wen-xiang^1,2, LIU Da-qun^1,2,3

1. Department of Plant Pathology, Agricultural University of Hebei, Baoding 071001, China;
2. Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China;
3. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China

Received:2015-02-20 Published:2015-06-28

摘要/Abstract

摘要： 为明确河农6251含有的抗叶锈基因并对其进行分子定位,以抗病品种河农6251与感病品种Thatcher的杂种F₁、F₂、F₃群体为材料,对河农6251的苗期抗叶锈基因进行定位。分子标记辅助鉴定结果表明,河农6251中含有抗叶锈基因 Lr26 和 LrZH84,可能含有 Lr2c 和 Lr17a;遗传分析表明,河农6251对叶锈菌PBGP的抗性由1对显性抗病基因决定,暂命名为 LrH6;用SSR技术和分离群体分组法(BSA)分析河农6251 F₂群体和F₃家系,结果位于1B染色体的3个SSR标记wmc419、wms582和barc120与 LrH6 连锁,遗传距离分别为21.6,25.8,27.9 cM。河农6251中含有 Lr26、LrZH84 等多个抗叶锈基因,其对PBGP的抗性由一对位于1BL染色体上的显性抗叶锈基因决定。

关键词: 小麦叶锈病, 抗病基因, SSR标记, 遗传分析, 遗传作图

Abstract: Preliminary research showed that they all contributed higher disease resistance level to Puccinia triticina.Detecting and mapping wheat leaf rust resistance gene were significance for application of the resistance in the wheat production.Resistant parents Henong 6251,susceptible parent Thatcher and their F₂,F₃ populations were employed to map the wheat leaf rust resistance genes in Henong 6251 based on SSR technique.Molecular marker-assisted identification results showed that Henong 6251 contained wheat leaf rust resistance genes Lr26 and LrZH84,and probably contained Lr2c and Lr17a.Henong 6251,Thatcher,and their F_l,F₂ populations and F₃ families were tested for detecting the wheat leaf rust resistance with single strain of Chinese P.triticina pathotype PBGP in greenhouse.The results showed that the wheat leaf rust resistance in Henong 6251 to PBGP was controlled by one dominant resistance gene,temporarily designated as LrH6.SSR molecular markers and BSA were used to test Henong 6251 and their resistant and susceptible bulks of F₂ and F₃populations for mapping the leaf rust resistance gene.Results indicated that Henong 6251 carried a single dominant resistance gene LrH6 to PBGP,and was preliminary located on chromosome 1B.The resistance gene LrH6 was linked to the three known SSR loci(wmc419,wms582 and barc120)on the 1BL with genetic distance ranging 21.6,25.8,27.9 cM respectively.Henong 6251 contained more than one leaf rust resistance genes including Lr26 and LrZH84 and the wheat leaf rust resistance to PBGP was controlled by one dominant and new resistance gene.

Key words: Wheat leaf rust, Resistance gene, SSR marker, Genetic analysis, Genetic mapping

中图分类号:

S435.121

任晓娣, 杜冬冬, 张河山, 姚宏鹏, 赵赛, 杨文香, 刘大群. 小麦河农6251苗期抗叶锈病基因的鉴定[J]. 华北农学报, 2015, 30(3): 192-199. doi: 10.7668/hbnxb.2015.03.033.

REN Xiao-di, DU Dong-dong, ZHANG He-shan, YAO Hong-peng, ZHAO Sai, YANG Wen-xiang, LIU Da-qun. Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 192-199. doi: 10.7668/hbnxb.2015.03.033.

http://www.hbnxb.net/CN/Y2015/V30/I3/192

参考文献

[1] Singh D,Mohler V,Park R F.Discovery,characterisation and mapping of wheat leaf rust resistance gene Lr71 [J].Euphytica,2013,190(1):131-136.
[2] 陈万权,秦庆明.国际上已知小麦抗叶锈病基因在中国的可利用性研究[J].中国农业科学,2002,35(7):794-801.
[3] 杨文香,孟庆芳,冯圣东,等.1999年我国小麦叶锈菌毒性监测[J].植物保护学报,2004,31(1):45-50.
[4] 陈万权,秦庆明,王奎荣,等.江苏省重要小麦品种抗叶锈病和秆锈病基因初步分析[J].植物保护学报,1997,24(3):225-234.
[5] 陈万权,王剑雄.76个小麦种质资源抗叶锈及秆锈基因初步分析[J].作物学报,1997,23(6):655-663.
[6] 陈万权,秦庆明,陈扬林,等.我国40个小麦品种抗叶锈性研究[J].植物病理学报,2001,31(1):16-25.
[7] 刘颖超,郭爱国,朱之堉,等.中国三麦区小麦抗叶锈基因部署研究[J].植物病理学报,1996,26(1):21-25.
[8] Li Z F,Xia X C,He Z H,et al.Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars[J].Plant Disease,2010,94(1):45-53.
[9] 师丽红,张娜,胡亚亚,等.10个小麦新品种(系)抗小麦叶锈性评价[J].中国农业科学,2011,44(14):2900-2908.
[10] 丁艳红,刘欢,师丽红,等.28个小麦微核心种质抗叶锈性分析[J].作物学报,2010,36(7):1126-1134.
[11] 赵丽娜,任晓娣,胡亚亚,等.23份中国小麦微核心种质抗叶锈性评价[J].中国农业科学,2013,46(3):441-450.
[12] Zhao X L,Zheng T C,Xia X C,et al.Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B[J].Theoretical and Applied Genetics,2008,117(7):1069-1075.
[13] Zhang H,Xia X C,He Z H,et al.Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16[J].Molecular Breeding,2011,28(4):527-534.
[14] 陈现朝,李星,李在峰,等.中国小麦贵州98-18中抗叶锈基因的分子定位[J].植物病理学报,2010,40(5):489-494.
[15] 李星,李在峰,李亚宁,等.小麦品系西农1163-4抗叶锈病基因的遗传分析和分子作图[J].中国农业科学,2010,43(12):2397-2402.
[16] Roelfs A P,Singh R P,Saari E E.Rust diseases of wheat:concepts and methods of disease management[J].Mexico,DF:CIMMYT,1992:7-14.
[17] 张娜,闫红飞,张英春,等.小麦抗叶锈病基因 Lr2c 的SSR标记[J].农业生物技术学报,2009,17(1):148-152.
[18] Herrera-Foessel S A,Djurle A,Yuen J,et al.Identification and molecular characterization of leaf rust resistance gene Lr14a in durum wheat[J].Plant Disease,2008,92(3):469-473.
[19] Mccartney C A,Somers D J,Mccallum B D,et al.Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BSc[J].Molecular Breeding,2005,15(4):329-337.
[20] Bremenkamp-Barrett B,Faris J D,Fellers J P.Molecular mapping of the leaf rust resistance gene Lr17a in wheat[J].Crop Science,2008,48(3):1124-1128.
[21] Hiebert C W,Thomas J B,Somers D J,et al.Microsatellite mapping of adult-plant leaf rust resistance gene Lr22a in wheat[J].Theoretical and Applied Genetics,2007,115(6):877-884.
[22] Singh A,Pallavi J K,Gupta P,et al.Identification of microsatellite markers linked to leaf rust resistance gene Lr25 in wheat[J].Journal of Applied Genetics,2012,53(1):19-25.
[23] Thomas J,Nilmalgoda S,Hiebert C,et al.Genetic markers and leaf rust resistance of the wheat gene Lr32 [J].Crop Science,2010,50(6):2310-2317.
[24] Hiebert C,Thomas J,Mccallum B.Locating the broad-spectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method[J].Theoretical and Applied Genetics,2005,110(8):1453-1457.
[25] Dadkhodaie N A,Karaoglou H,Wellings C R,et al.Mapping genes Lr53 and Yr35 on the short arm of chromosome 6B of common wheat with microsatellite markers and studies of their association with Lr36 [J].Theoretical and Applied Genetics,2011,122(3):479-487.
[26] Hiebert C W,Thomas J B,Callum B C.Gnetic mapping of the wheat leaf rust resistance gene Lr60 (LrW2) [J].Crop Science,2008,48(3):1020-1026.
[27] Kolmer J A,Anderson J A,Flor J M.Chromosome location,linkage with simple sequence repeat markers,and leaf rust resistance conditioned by gene Lr63 in wheat[J].Crop Science,2010,50(6):2392-2395.
[28] Mohler V,Singh D,Singruen C,et al.Characterization and mapping of Lr65 in spelt wheat Altgold Rotkorn[J].Plant Breeding,2012,131(2):252-257.
[29] Herrera-Foessel S A,Lagudah E S,Huerta-Espino J,et al.New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked[J].Theoretical and Applied Genetics,2011,122(1):239-249.
[30] Herrera-Foessel S A,Singh R P,Huerta-Espino J,et al. Lr68 :a new gene conferring slow rusting resistance to leaf rust in wheat[J].Theoretical and Applied Genetics,2012,124(8):1475-1486.
[31] Singh D,Mohler V,Park R F.Discovery,characterisation and mapping of wheat leaf rust resistance gene Lr71 [J].Euphytica,2013,190(1):131-136.
[32] Zhou Y,Xia X,He Z H,et al.Fine Mapping of leaf rust resistance gene LrZH84 using expressed sequence tag and sequence-tagged site markers,and allelism with other genes on wheat chromosome 1B[J].Phytopathology,2013,103(2):169-174.
[33] Dyck P L,Kerber E R,Lukow O M.Chromosome location and linkage of a new gene (LR33) for reaction to puccinia-recondita in common wheat[J].Genome,1987,29(3):463-466.
[34] Dyck P L,Sykes E E.Genetics of leaf-rust resistance in three spelt wheats[J].Can J Plant Science,1994,74(2):231-233.

选择文件类型/文献管理软件名称

选择包含的内容

小麦河农6251苗期抗叶锈病基因的鉴定

Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	凌晨, 赵洪, 颜军, 马莹雪, 冯艳芳, 张靖立, 李波, 邓超, 徐振江. 基于SSR标记的莴苣品种鉴定体系的建立及应用[J]. 华北农学报, 2022, 37(4): 34-44.
[2]	王佳荣, 董瑞, 张梦宇, 高璞, 张培培, 李在峰, 刘大群. 40份CIMMYT小麦品系苗期及成株抗叶锈病基因鉴定[J]. 华北农学报, 2022, 37(2): 201-210.
[3]	董丽, 石海春, 赵长云, 余学杰, 柯永培. 玉米矮秆突变体K718d的遗传鉴定[J]. 华北农学报, 2021, 36(6): 71-77.
[4]	姚佳延, 于国琦, 洪益, 吕超, 许如根. 大麦株高类性状的遗传分析与QTL定位[J]. 华北农学报, 2021, 36(2): 28-32.
[5]	高天一, 郝芳敏, 臧全宇, 马二磊, 黄芸萍, 王毓洪. 基于SLAF-seq及BSA技术的甜瓜抗蔓枯病QTL定位分析[J]. 华北农学报, 2021, 36(2): 40-45.
[6]	伊六喜, 萨如拉, 王树彦, 李志伟, 斯钦巴特尔, 李强. 亚麻木酚素含量的SSR关联分析[J]. 华北农学报, 2021, 36(1): 63-70.
[7]	吕冰冰, 代畅, 彭正松, YAMAMOTO Naoki, 吴一超, 魏淑红, 杨在君. 一组新的小麦EST-SSR标记开发及其在遗传图谱构建中的应用[J]. 华北农学报, 2020, 35(4): 57-63.
[8]	邳瑞雪, 杜亮, 李娜, 栾维江, 李云海. *拟南芥突变体sus40-1D的遗传分析与候选基因鉴定*[J]. 华北农学报, 2020, 35(3): 63-68.
[9]	石海春, 陈立, 李开兵, 余学杰, 袁继超, 曲比伍合, 柯永培. 玉米矮秆突变体K125d的遗传鉴定[J]. 华北农学报, 2020, 35(1): 65-72.
[10]	李祥栋, 石明, 陆秀娟, 潘虹, 魏心元, 陆平. 利用叶绿体基因组SSR标记揭示薏苡属种质资源的遗传多样性[J]. 华北农学报, 2019, 34(S1): 6-14.
[11]	郭慧娟, 贾举庆, 李欣, 乔麟轶, 阎晓涛, 任永康, 常利芳, 张树伟, 畅志坚, 张晓军. 小麦种质CH7015中抗白粉病基因的SSR定位[J]. 华北农学报, 2019, 34(6): 203-208.
[12]	张冰冰, 刘霞, 秦梦凡, 梁峰豪, 张燕, 左凯峰, 郭娜, 马宁, 黄镇, 徐爱遐. 甘蓝型油菜开花时间的遗传分析及相关分子标记[J]. 华北农学报, 2019, 34(6): 39-46.
[13]	张敬敬, 张海英, 潘秀清, 许勇, 任毅, 高秀瑞, 李冰, 史宇凡, 党继革, 杨明珠, 武彦荣. 河北省130份西瓜品种与种质资源抗病基因KASP检测分析[J]. 华北农学报, 2019, 34(2): 110-116.
[14]	贾小平, 张博, 李剑峰, 袁玺垒, 陆平, 戴凌峰. 谷子SSR标记与光周期敏感性的关联分析[J]. 华北农学报, 2019, 34(1): 89-96.
[15]	白晓倩, 于澎湃, 李延玲, 高建明, 裴忠有, 罗峰, 孙守钧. 粒用高粱F₂群体农艺性状数量遗传分析[J]. 华北农学报, 2019, 34(1): 107-114.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

小麦河农6251苗期抗叶锈病基因的鉴定

Identification of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价