摘要： 为了创新蓖麻的种质资源,以通篦5号为材料,用0,0.02,0.03,0.04,0.05,0.06 mol/L的EMS溶液处理去壳的蓖麻种子,处理时间分别设定4,8,12 h,研究不同处理浓度和时间组合对蓖麻种子发芽率和根长的影响。结果表明:用0.04 mol/L的EMS溶液处理4 h,蓖麻种子的相对发芽率为49%,为半致死剂量处理组合,平均主根长10.45 mm。随着处理浓度的增加和处理时间的延长,蓖麻种子的相对发芽率和平均根长均表现下降趋势,处理浓度到0.06 mol/L时,蓖麻种子完全不能萌发;对10个突变体ISSR分子标记分析发现,M2发生了分子水平的变异,引物P91扩增出的多态性位点达13个。
关键词:
蓖麻,
EMS,
半致死剂量,
分子标记
Abstract: In order to innovate castor germplasm,hulled castor seeds were treated with EMS at 6 levels (0,0.02,0.03,0.04,0.05,0.06 mol/L)and different time(4,8,12 h),The results showed that lethal concentration 50 was at 0.04 mol/L and 4 h,the average taproot length was 10.45 mm,and the concentration of EMS reached to 0.06 mol/L,the seeds could not germinate completely.With the increasing of treatment time and concentration,castor seed germination rate and average relative root length showed a downward trend;ISSR molecular marker analysis showed that DNA variation existed in M2 by EMS.The primer P91 detected 13 variation loci.
Key words:
Castor,
EMS,
Median lethal dose,
Molecular marker
中图分类号:
刘鹏, 李旭新, 张春兰, 张园园, 张卫国. 蓖麻EMS处理条件分析及突变体分子标记检测[J]. 华北农学报, 2015, 30(4): 47-50. doi: 10.7668/hbnxb.2015.04.009.
LIU Peng, LI Xu-xin, ZHANG Chun-lan, ZHANG Yuan-yuan, ZHANG Wei-guo. Analysis of EMS Treatment Conditions on Castor and Mutants are Detected by Molecular Marker[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 47-50. doi: 10.7668/hbnxb.2015.04.009.