TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究

doi:10.7668/hbnxb.2008.S2.019

华北农学报 ›› 2008, Vol. 23 ›› Issue (S2): 88-91. doi: 10.7668/hbnxb.2008.S2.019

所属专题： 畜牧；

TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究

温立斌¹, 何孔旺¹, 杨汉春², 王玉然³, 董美响⁴

1. 江苏省农业科学院, 兽医研究所, 江苏, 南京, 210014;
2. 中国农业大学, 动物医学院, 北京, 100094;
3. 河北省动物卫生监督所, 河北, 石家庄, 050081;
4. 河北省饲料工业协会, 河北, 石家庄, 050011

收稿日期:2008-08-30 出版日期:2008-12-31
作者简介:温立斌(1967-),男,河北宣化人,博士,高级兽医师,主要从事动物分子病毒学与免疫学研究.
基金资助:
国家“973”计划前期研究专项资助课题(2007CB116308);江苏省博士后基金(5910602)

Apoptosis in Immune Organs of Pigs Induced by Porcine Circovirus Type 2-like Agent P1

WEN Li-bin¹, HE Kong-wang¹, YANG Han-chun², WANG Yu-ran³, DONG Mei-xiang⁴

1. Veterinary Research Insititute, Jiangsu Academy of Agriculture Science, Nanjing 210014, China;
2. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;
3. Animal Health Supervision Institute of Hebei Province, Shijiazhuang 050081, China;
4. Hebei Feed Industry Association, Shijiazhuang 050011, China

Received:2008-08-30 Published:2008-12-31
Supported by:
>

摘要/Abstract

摘要： 研究类猪圆环病毒2型因子P1体内诱导的细胞凋亡作用。将P1分子克隆接种普通仔猪,用原位末端标记技术(TUNEL)检测猪的扁桃体、脾脏、腹股沟淋巴结等免疫器官细胞的凋亡情况。猪感染P1后21和35 d的扁桃体、脾脏和腹股沟淋巴结等免疫器官细胞出现了凋亡。P1可能引起猪的免疫抑制。

关键词: 猪圆环病毒2型, P1, 凋亡, TUNEL

Abstract: The research is to study the apoptosis of pigs induced by porcine circovirus type 2??like agent P1. Theapoptosis was detected by TUNEL assay in immune organ samples( tonsil, spleen, superficial inguinal lymph node) of pigsinfected by P1 molecular clone.The results showed that the apoptosis??specific signal was detected in immune organ sam??ples( tonsil, spleen, superficial inguinal lymph node) at 21 and 35 d after P1 infection.The result suggests that P1 appearsto be related to immunosuppression resulted in apoptosis of lymphocyte.

Key words: Porcine circovirus type 2, P1, Apoptosis, TUNEL

中图分类号:

S852.65

温立斌, 何孔旺, 杨汉春, 王玉然, 董美响. TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究[J]. 华北农学报, 2008, 23(S2): 88-91. doi: 10.7668/hbnxb.2008.S2.019.

WEN Li-bin, HE Kong-wang, YANG Han-chun, WANG Yu-ran, DONG Mei-xiang. Apoptosis in Immune Organs of Pigs Induced by Porcine Circovirus Type 2-like Agent P1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 88-91. doi: 10.7668/hbnxb.2008.S2.019.

http://www.hbnxb.net/CN/Y2008/V23/IS2/88

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TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究

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TUNEL法检测类猪圆环病毒2型因子P1诱导猪免疫器官细胞凋亡的研究

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