摘要： 以砂珍棘豆(Oxytropis racemosa Turcz。)DNA为材料，应用分子标记技术，分析了DNA模板浓度、Mg²⁺浓度、引物浓度和dNTP浓度对ISSR-PCR扩增结果的影响，经优化试验建立了砂珍棘豆ISSR-PCR反应体系。20.μL PCR反应液含有的组分和终浓度分别为：20.ng DNA模板，1 U Taq酶，0.24 μmol/L引物，2.0.mmol/L MgCl₂，0.15 mmol/L dNTPs。

关键词: 砂珍棘豆, ISSR, PCR, 优化

Abstract: Several factors that affected the ISSR-PCR applification ofO.racemosaTurcz.,such as concentration of template DNA,primer,Mg2+^{and dNTP were examined.The optimal ISSR-PCR reaction system in the experiment was established as follows:20 ng template DNA,0.24μmol/L primer,2.0 mmol/L MgCl_{2<.sub>and 0.15 mmol/L dNTPs.The research provided some foundamental data to the studies and use ofO.racemosaTurcz}}

Key words: Oxytropis racemosaTurcz., ISSR, PCR, Optimization

中图分类号: 

• Q346