摘要： 利用本研究小组克隆的猪IgG Ⅱ类Fc受体cDNA(swFcγRⅡ)基因序列(DQ02606)设计引物，应用RT-PCR技术，从猪外周血白细胞cDNA中扩增了完整的960 bp swFcγRⅡ ORF序列，利用基因重组技术将swFcγRⅡ ORF基因亚克隆到真核表达载体pcDNA3，经PCR及双酶切鉴定：扩增出901 bp的PCR产物；Kpn I/EcoR I双酶切，切出5376和901bp DNA片段，表明重组质粒pcDNA/swFcγRⅡ构建成功。然后脂质体法转染COS-7细胞，用玫瑰花环试验鉴定了swFcγRⅡ配体亲和特性。

关键词: 猪, IgG Ⅱ类Fc受体, 表达载体, 玫瑰花环试验

Abstract: The Fc receptor cDNA sequence of porcine IgG II cloned by us was used to design primers. Using RT-PCR technology, a 960 bp of swFcγRII sequence covering the whole coding region was amplified from the peripheral blood leucocyte cDNA, and the was sub-cloned into the expression vector pcDNA3. Through double digestion by Kpn I/EcoR I and PCR identification, a 5375 bp and a 901 bp of DNA fragments were obtained, indicating that the recombinant plasmid pcDNA/swFcγRII was successfully constructed. The 901 bp fragment was then transfected into COS-7 cells by the lipofection method, and the ligand affinity of swFcγRII was identified by rosetting procedures.

Key words: Pig, Fc-RII, Express edvector, Rosetting

中图分类号: 

• S828.03