摘要： 基于电子延伸序列，本试验克隆并分析了鸡Muslin基因。肌肉组织提取总RNA，利用设计的引物进行RT-PCR，PCR产物与pMD19-T连接后转化E。coli DH5α，检测阳性克隆并测序；克隆的Musclin基因与人、大鼠、小鼠同源性分别为74%，7%，70%，推测的氨基酸序列同源性分别为58%，54%，50%，氨基酸序列含有“KKKR”结构和与小鼠ANP，BNP，CNP蛋白的同源性区域。试验成功克隆了鸡Musclin基因并注册GenBank(EF551041)。

关键词: 鸡, Musclin, 克隆, 序列分析

Abstract: The coding sequence of chicken Musclin gene was amplified based on the in silico sequence information in the present study. Total RNA was extracted from muscle tissue and mRNA sequence of gene was amplified by RT-PCR using designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of E. coli DH5α. The sequence was analyzed to identify the recombinant plasmid. Identity analysis show that the chicken Musclin nucleotide sequence share 74%, 73% and 70% and the deduced protein sequence share 58%, 54% and 50% homology with that of human, rat and mouse, respectively. The deduced peptide contained a "KKKR" motif and the region homologous to mouse ANP, BNP, CNP. The chicken Musclin gene was successfully cloned in the present study and the sequence has been submitted to GenBank (EF551041).

Key words: Chicken, Musclin, cDNA Cloning, Sequence analysis

中图分类号: 

• S811.3

• Q785