摘要： 采用根癌农杆菌感染半夏无菌叶片、叶柄和愈伤组织，对GUS基因的瞬间表达进行研究，并分析了不同转化受体、感染时间、菌液浓度、共培养、预培养时间、菌种对GUS基因的瞬间表达的影响。结果显示，以半夏的无菌苗叶柄和愈伤组织，在OD值为0.2的EHA101或EHA105农杆菌液中感染15 min，叶柄暗处共培养3 d，愈伤组织2 d，能够得到较高的GUS基因瞬间表达。
关键词:
半夏,
根癌农杆菌,
GUS基因,
瞬间表达
Abstract: Young leaves, petioles and calli of Pinellia ternata Breit were transformed with GUS gene via Agrobacterium tumefaciens. The effects of different explants, infection period, concentration of Agrobacterium tumefaciens, days of co-culture and pre-culture, and strains on the transient expression of GUS gene were analyzed. The result indicated that a higher transient express rate of GUS gene could be acquired from the explant petioles and calli which were dipped into Agrobaterium suspension EHA102 or EHA105 (OD600 adjusted to 0.2) for 15 min, and co-cultured for 3 days and 2 days, respectively, in darkness.
Key words:
Pinellia ternata Breit,
Agrobacterium tumefaciens,
GUS gene,
Transient express
中图分类号:
贾永芳, 马玉坤, 郭余龙, 李名扬. 农杆菌介导的半夏GUS基因瞬时表达[J]. 华北农学报, 2007, 22(4): 42-45. doi: 10.7668/hbnxb.2007.04.010.
JIA Yong-fang, MA Yu-kun, GUO Yu-long, LI Ming-yang. Research on the Transient Express of GUS Gene by Agrobacterium tumefaciens in Pinellia ternata Breit[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 42-45. doi: 10.7668/hbnxb.2007.04.010.