摘要： 1-氨基环丙烷-1-羧酸合成酶（ACS）是高等植物中乙烯合成的关键酶。以成熟河套蜜瓜果实的RNA为模板，经反转录和PCR扩增合成和克隆了河套蜜瓜ACS基因cDNA片断（627 bp），将ACS基因cDNA反向构建到植物表达载体pGA643，配制成DNA溶液后用花粉管通道法导入外源基因，对转基因河套蜜瓜的叶片用CTAB法提取总DNA，PCR扩增检测，结果表明某些果实的部分种子已整合外源基因。目前，该转基因新品系正在进行生态适应性研究及“环境释放”和“生产性试验”的安全性评价。

关键词: ACS基因, PCR扩增技术, 花粉管通道法, CTAB法, 生态适应性

Abstract: 1-Aminocyclopropane-1-carboxylic acid synthase(ACS) is the key enzyme in the biosynthasis of ethylene.Total RNA was isolated from ripe Hetao melon fruits.Using oligo(dT)15 as primer,the fruits strand of ACC cDNA was synthesized and a 627 bp DNA fragment was obtained with PCR technique.This ACC cDNA fragment was cloned into site of vector pGA643.Using the pollen-tube pathway to introduce exogenetic genes,then total DNA was isolated from the genetically modified Hetao melon by CTAB method,and was detected for PCR amplification,the result indicated that some seeds had integrated exogenetic genes.Presently,the new strain which had been genetically modified was being done environmental release and generative test.

Key words: ACC gene, PCR amplification technique, Pollen-tube pathway, CTAB method, Ecological

中图分类号: 

• S652.603.2