高效提取越橘成熟组织基因组DNA的方法

doi:10.7668/hbnxb.2008.S2.047

华北农学报 ›› 2008, Vol. 23 ›› Issue (S2): 205-208. doi: 10.7668/hbnxb.2008.S2.047

所属专题： 生物技术；

高效提取越橘成熟组织基因组DNA的方法

张鲁杰, 夏秀英, 徐娜, 贺建华, 徐品三

大连理工大学, 环境与生命学院, 辽宁, 大连, 116024

收稿日期:2008-09-18 出版日期:2008-12-31
通讯作者: 夏秀英( 1972- ), 女, 黑龙江庆安人, 副教授, 硕士生导师, 主要从事植物基因工程研究。
作者简介:张鲁杰(1987-),女,山东济宁人,在读硕士,主要从事植物分子生物学研究.

An Efficient Method for Genomic DNA Extraction from Mature Tissues of Blueberry(Vaccinium spp.)

ZHANG Lu-jie, XIA Xiu-ying, XU Na, HE Jian-hua, XU Pin-san

College of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China

Received:2008-09-18 Published:2008-12-31

摘要/Abstract

摘要： 为从越橘成熟组织中提取高质量DNA,对CTAB法进行改良,并与常规CTAB法及快速CTAB法的提取效果进行比较。结果表明:改良方法提取获得的DNA产量大、纯度高,DNA产量平均为192μg/g,OD260/OD280在1.75~1.85之间,所得DNA能被限制性内切酶完全消化,以其为模板进行SRAP-PCR扩增,能得到清晰、稳定的条带,证明改良方法适于越橘成熟组织DNA的提取。

关键词: 越橘, 成熟叶片, DNA提取, 改良CTAB法, SRAP

Abstract: To obtain high quality genomic DNA from mature tissues of blueberry( Vaccinium spp. ), a modif ied CTAB method was established.And then three different methods, the conventional CTAB method, rapid CTAB method and modi??fied CTAB method, were compared. The results showed that the modified method could obtain the expecting DNA thatreached the higher purity (OD260/OD280 is between 1. 75 and 1. 85) and higher yield ( 192 g/ g). Furthermore, the DNA can be digested completely with restriction endonuclease, and the electrophoresis bands of SRAP??PCR were clear and stable. It is concluded that this modified CTAB method should be suitable for extraction of genomic DNA from mature tissuesof blueberry( Vaccinium spp. ).

Key words: Blueberry( Vaccinium spp. ), Mature leaves, Genomic DNA extraction, Modified CTAB, SRAP

中图分类号:

S666.2

张鲁杰, 夏秀英, 徐娜, 贺建华, 徐品三. 高效提取越橘成熟组织基因组DNA的方法[J]. 华北农学报, 2008, 23(S2): 205-208. doi: 10.7668/hbnxb.2008.S2.047.

ZHANG Lu-jie, XIA Xiu-ying, XU Na, HE Jian-hua, XU Pin-san. An Efficient Method for Genomic DNA Extraction from Mature Tissues of Blueberry(Vaccinium spp.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 205-208. doi: 10.7668/hbnxb.2008.S2.047.

http://www.hbnxb.net/CN/Y2008/V23/IS2/205

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高效提取越橘成熟组织基因组DNA的方法

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