华北农学报 ›› 2013, Vol. 28 ›› Issue (2): 91-95. doi: 10.3969/j.issn.1000-7091.2013.02.017

所属专题: 小麦

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低磷对小麦代换系幼苗根系保护酶活性和丙二醛含量的影响及染色体效应

米少艳1, 靖姣姣1, 白志英1, 李存东2   

  1. 1. 河北农业大学 生命科学学院, 河北 保定 071000;
    2. 河北农业大学 农学院, 河北 保定 071000
  • 收稿日期:2012-01-15 出版日期:2013-04-28
  • 通讯作者: 白志英(1967-),女,河北正定人,教授,博士生导师,主要从事植物资源利用与开发研究。李存东(1964-),男,河北清河人,教授,博士生导师,主要从事作物生理生态研究。
  • 作者简介:米少艳(1987-),女,河北临城人,在读硕士,主要从事植物资源利用与开发研究。
  • 基金资助:
    河北省自然科学基金项目(C2011204016)

Effects of Phosphorus Deficiency Stress on Protective Enzyme Activities, MDA Content and Chromosome of Wheat Substitution Lines Seedling Roots

MI Shao-yan1, JING Jiao-jiao1, BAI Zhi-ying1, LI Cun-dong2   

  1. 1. College of Life Science, Hebei Agricultural University, Baoding 071000, China;
    2. College of Agronomy, Hebei Agricultural University, Baoding 071000, China
  • Received:2012-01-15 Published:2013-04-28

摘要: 以中国春-Synthetic 6x染色体代换系及其亲本为材料,通过测定不同磷处理条件下根系抗氧化酶SOD、POD活性和丙二醛(MDA)含量,研究低磷胁迫对小麦代换系酶活性的影响,并对耐低磷胁迫特性的基因进行染色体定位。结果表明,低磷胁迫下,小麦代换系苗期根系SOD和POD活性显著升高,丙二醛含量降低。Synthetic 6x的3A、4A、5A和7A染色体上可能存在诱导根系SOD活性增强的基因,5A、1D和2D染色体上可能存在诱导根系POD活性增强的基因,1A、2A、4B、6B和7D染色体上可能存在抑制根系MDA含量增高的基因。

关键词: 小麦代换系, 根系, 低磷, 保护酶, 染色体效应

Abstract: The effects of phosphorus deficiency stress on enzyme activity in wheat was studied by determining antioxidant enzymes SOD,POD activities and MDA content and locating thegene controlling antioxidant enzymes SOD,POD activities and MDA content at the roots of the seedling stage using wheat substitution lines between Chi-nese Spring(CS)and Synthetic 6x. The results showed that SOD and POD activities increased,MDA content reduced under phosphorus deficiency stress. This explains that the increased of antioxidant enzyme activity might reduce MDA content and lower membrane lipid peroxidation level,to improve their resistance. Thegenes promoting SOD activity might be located on 3A,4A,5A and 7A chromosome of Synthetic 6x;and thegenes promoting POD activity might be located on 5A,1D and 2D chromosome,while thegenes inhibiting content might be located on 5A,2D,5D and 7D chromosome of Synthetic 6x.

Key words: Substitution lines, Roots, P-deficiency, Protective enzymes, Chromosome effect

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引用本文

米少艳, 靖姣姣, 白志英, 李存东. 低磷对小麦代换系幼苗根系保护酶活性和丙二醛含量的影响及染色体效应[J]. 华北农学报, 2013, 28(2): 91-95. doi: 10.3969/j.issn.1000-7091.2013.02.017.

MI Shao-yan, JING Jiao-jiao, BAI Zhi-ying, LI Cun-dong. Effects of Phosphorus Deficiency Stress on Protective Enzyme Activities, MDA Content and Chromosome of Wheat Substitution Lines Seedling Roots[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 91-95. doi: 10.3969/j.issn.1000-7091.2013.02.017.

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