基于SRAP的小麦籽粒性状QTL定位

doi:10.3969/j.issn.1000-7091.2012.05.014

华北农学报 ›› 2012, Vol. 27 ›› Issue (5): 72-79. doi: 10.3969/j.issn.1000-7091.2012.05.014

所属专题： 小麦；

基于SRAP的小麦籽粒性状QTL定位

李清峰, 马守才, 王志军, 史秀秀, 毕晓静, 陈晓文, 张改生

西北农林科技大学国家杨凌农业生物技术育种中心国家小麦改良中心杨凌分中心小麦育种教育部工程研究中心陕西省作物杂种优势研究与利用重点实验室

收稿日期:2012-07-17 出版日期:2012-10-28
通讯作者: 马守才(1968-)，男，陕西大荔人，副教授，博士，硕士研究生导师，主要从事小麦杂种优势利用与种子工程研究
作者简介:李清峰(1984-)，男，安徽界首人，硕士，主要从事小麦杂种优势利用研究。
基金资助:
“863”计划子课题(2009AA101102);陕西省自然科学基金项目(2012JM3003)

Locating the Quantitative Trait Loci(QTL) of Wheat Yield-related Traits Based on SRAP Markers

LI Qing-feng, MA Shou-cai, WANG Zhi-jun, SHI Xiu-xiu, BI Xiao-jing, CHEN Xiao-wen, ZHANG Gai-sheng

Northwest A&F University National Yangling Agricultural Biotechnology and Breeding Center Yangling Branch of State Wheat Improvement Center Wheat Breeding Engineering Research Center Ministry of Education Key Laboratory of Crop Heterosis of Shaanxi Province Yangling 712100 China

Received:2012-07-17 Published:2012-10-28

摘要/Abstract

摘要： 籽粒大小和形态性状影响到小麦的产量和磨粉品质。为了鉴定小麦籽粒性状相关的数量性状位点(QTL),以大粒品种西农817为母本和小粒品种中国春为父本及其F2群体为试验材料,分析籽粒长度、宽度、厚度及千粒质量的性状表现,并利用SRAP结合锚定标记SSR构建的遗传图谱,对这4个性状进行QTL定位分析。共检测到50个影响籽粒长度、宽度、厚度及千粒质量的QTLs,这些QTL分布在1A、2A、2D、3B、4B、4D、5D、6A、6D、7A染色体和5个连锁群。在检测到的QTLs中,与籽粒长度、宽度、厚度和千粒质量相关的QTL分别为16,13,10和11个。

关键词: 小麦籽粒性状, SRAP和SSR, QTL定位

Abstract: Grain size and morphology influence the yield and milling quality of bread wheat.The objective of this study was to identify quantitative trait loci(QTL) controlling kernel traits in wheat.Using a F2 population derived from Chinese winter wheat varieties Xinong 817 and China spring,four agronomic traits of grain length(GL),grain width(GW),grain thickness(GT) and 1000-grain weight(TGW) were evaluated.Based on F2 population,a genetic map comprising SRAP markers and SSR anchor markers was generated.According to the genetic map and phenotypic data,quantitative trait loci(QTL) were located for these agronomic traits using the inclusive composite interval mapping(ICIM) method.A total of 50 QTLs involving 1A,2A,2D,3B,4B,4D,5D,6A,6D and 7A wheat chromosomes and 5 linkage groups were identified for these four traits.Among them,16,13,10 and 11 QTLs for GL,GW,GT and TGW were identified,respectively.In addition,15 genomic regions with plenty of QTLs were detected on 1A,2D,4D,5D,6A,7A and 4 linkage groups in this study.

Key words: Wheat yield-related traits, SRAP and SSR, Quantitative trait loci

中图分类号:

S512.1

李清峰, 马守才, 王志军, 史秀秀, 毕晓静, 陈晓文, 张改生. 基于SRAP的小麦籽粒性状QTL定位[J]. 华北农学报, 2012, 27(5): 72-79. doi: 10.3969/j.issn.1000-7091.2012.05.014.

LI Qing-feng, MA Shou-cai, WANG Zhi-jun, SHI Xiu-xiu, BI Xiao-jing, CHEN Xiao-wen, ZHANG Gai-sheng. Locating the Quantitative Trait Loci(QTL) of Wheat Yield-related Traits Based on SRAP Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 72-79. doi: 10.3969/j.issn.1000-7091.2012.05.014.

http://www.hbnxb.net/CN/Y2012/V27/I5/72

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