甜瓜果肉β-胡萝卜素含量的QTL分析

doi:10.3969/j.issn.1000-7091.2012.05.015

摘要/Abstract

摘要： β-胡萝卜素的QTL定位为开展相关基因克隆,应用分子标记辅助育种培育高品质甜瓜品种奠定基础。应用2种果肉颜色差异较大的甜瓜品种200930与200932杂交得到的F2群体构建富含β-胡萝卜素含量特征的遗传图谱。构建的基因图包含154对SSR标记,其中77对标记是由EST序列新开发的EST-SSR引物。该图谱包括17条连锁群,覆盖基因组长度1 663.5 cM,标记间的平均距离为10.8 cM。定位到3个与β-胡萝卜素含量有关的QTL位点,分别位于第1,4(Chr4),7(Chr7)连锁群上,2个QTL贡献率超过15%。位于第1连锁群的QTLβ-car1贡献率最大,为26.9%。

关键词: 甜瓜, SSR标记, &beta, -胡萝卜素, 遗传图谱, QTL

Abstract: QTL mapping of the β-carotene was useful for relative gene cloning and the molecular assisted breeding to cultivate high-quality melon.A genetic map enriched for β-carotene content was constructed,using a F2 population developed from a cross between two species which have great differences in fruit color：200930 and 200932.The map included 154 SSR markers,of which 77 were newly developed from fruit EST.The genetic map consisted of 17 linkage groups spanning 1 663.5 cM with an average of 10.8 cM between markers.Total three QTLs for β-carotene content were detected.These QTLs were mapped on linkage groups 1,4(Chr4),7(Chr7) respectively.Two QTLs explained phenotypic variation more than 15%.The QTL β-car1 which was mapped on the first linkage group explained up to 26.9% of the phenotypic variances.

Key words: Cucumis melo L., SSR markers β-carotene, Genetic map QTL

中图分类号:

S652

吕丽华, 王怀松, 付秋实, 朱慧芹. 甜瓜果肉β-胡萝卜素含量的QTL分析[J]. 华北农学报, 2012, 27(5): 80-85. doi: 10.3969/j.issn.1000-7091.2012.05.015.

Li-hua, WANG Huai-song, FU Qiu-shi, ZHU Hui-qin. QTL Analysis of β-carotene Content inCucumis melo L. Flesh[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 80-85. doi: 10.3969/j.issn.1000-7091.2012.05.015.

http://www.hbnxb.net/CN/Y2012/V27/I5/80

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