光质对番茄果实表面光系统活性的影响

doi:10.3969/j.issn.1000-7091.2012.01.028

摘要/Abstract

摘要： 以保罗塔番茄果实为试材,采用叶绿素成像荧光仪(MINI-IMAGING-PAM) 测试分析了蓝(453±2) nm、绿(519±2) nm、红(623±2) nm 3种光质对番茄果实表面光化学活性的影响。结果表明:随着光强的增加(200～2 500μmol/(m²·s)),蓝光和红光处理番茄果实表面光系统Ⅱ(PSII) 最大光化学量子产量Fv/Fm、PSII实际量子产量Y(Ⅱ) 、电子传递速率ETR和PSII天线转化效率Fv'/Fm'均呈"S"型下降趋势;光系统间激发能分配不平衡偏离系数β/α-1急剧上升,反映出高光强导致光系统间激发能分配的不平衡,PSⅡ和PSⅠ间线性电子传递的协调性降低;而绿光处理番茄果实表面叶绿素荧光参数相对来说变化不大,可能是绿光减少了叶绿素对光能的过度吸收,使得光抑制程度较轻;蓝光处理番茄果实表面Fv/Fm、Y(Ⅱ) 、ETR和Fv'/Fm'均小于红光和绿光处理,并且随着光强的增加这种差距越来越明显,表明蓝光对光抑制更敏感,更容易受到强光胁迫的影响;另外,当光强为200～1 000μmol/(m²·s) 时,番茄果实表面Fv/Fm、Y(Ⅱ) 和Fv'/Fm'值红光>绿光,超过1 000μmol/(m²·s) 时,则绿光>红光,反映出绿光处理番茄果实适应强光的能力较强。

关键词: 番茄, 果实, 光质, 叶绿素荧光

Abstract: The effect of light quality on the photochemical activity of tomato fruit surface was studied by a MINI-IMAGING-PAM system. The results showed that as the intensity of light quality rises(200-2 500 μmol/(m²·s)), the maximal photochemical efficiency(Fv/Fm), the effective PS II quantum yield(Y(II)), the apparent electron transport rate(ETR) and the open PSII reaction centers(Fv'/Fm') of tomato fruit surface which was under red light and blue light treatments all decreased in the shape of " S", while the relative deviation(β/α-1) from full balance between two photosystems rised gradually, indicating that excitation energy high intensity has broken the balance of excitation energy distribution between PSII and PSI, thus leading to the poorer cooperation of two photosystems. Chlorophyll fluorescence of tomato fruit surface which was under green light treatments was proved to be feasible, showing that green light may reduce the chlorophyll to absorb excessive of light energy and decrease photoinhibition. Fv/Fm、Y(Ⅱ) 、ETR and Fv'/Fm'of tomato fruit surface which was under blue light was smaller than red light and blue light, these gaps will widen with an increase in intensity, which indicated blue light was sensitive to photoinhibition. In case of lower light intensity(200-1 000 μmol/(m²·s)), Fv/Fm、Y(Ⅱ) and Fv'/Fm'of tomato fruit surface which was under red light was higher than green light, while over 1 000 μmol/(m²·s) leading to reverse results, which showed that the capacity of adapting to high light intensity of tomato fruit surface which was under green light was strong.

Key words: Tomato, Fruit, Light qualitative, Chlorophyll fluorescence

中图分类号:

S641.01

王俊玲, 李晓峰, 王梅, 高志奎, 霍捷. 光质对番茄果实表面光系统活性的影响[J]. 华北农学报, 2012, 27(1): 151-154. doi: 10.3969/j.issn.1000-7091.2012.01.028.

WANG Jun-ling, LI Xiao-feng, WANG Mei, GAO Zhi-kui, HUO Jie. Effects of Light Quality on the Photosystem Activity of Tomato Fruit Surface[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 151-154. doi: 10.3969/j.issn.1000-7091.2012.01.028.

http://www.hbnxb.net/CN/Y2012/V27/I1/151

参考文献

[1] 蒲高斌,刘世琦,刘磊,等.不同光质对番茄幼苗生长和生理特性的影响[J] 园艺学报,2005,32(3):420-425.
[2] 杜洪涛,刘世琦,张珍,等.光质对彩色甜椒幼苗生长及酶活性影响[J] 华北农学报,2005,20(2):45-48.
[3] 刘立侠.光质对人参叶绿体结构和光合生理性状的影响[J] 植物学报,1993,25(8):588-592.
[4] 杜洪涛,刘世琦,蒲高斌,等.光质对彩色甜椒幼苗生长及叶绿素荧光特性的影响[J] 西北农业学报,2005,14(1):41-45.
[5] 徐凯,郭延平,张上隆.不同光质对草莓叶片光合作用和叶绿素荧光的影响[J] 中国农业科学,2005,38(2):369-375.
[6] Voskresenskaya N P,Nechayeva E P,Vlasova M P,et al.Thesignificance of blue light and kinetin for restoration of thephotosynthetic apparatus in aging narley leaves[J] Fizid Rast,1968,15:890.
[7] 马建忠,娄世庆,匡廷云,等.不同光质对黍子(Panicum miliaceum) 叶绿体光系统发育及psbA 基因转录物稳态含量的影响[J] 遗传学报,1997,24(5):464-470.
[8] 王绍辉,孔云,陈青君,等.不同光质补光对日光温室黄瓜产量与品质的影响[J] 中国生态农业学报,2006,14(4):119-121.
[9] 倪纪恒,陈学好,陈春宏,等.补充不同光质对温室黄瓜生长发育、光合和前期产量的影响[J] 中国农业科学,2009,42(7):2615-2623.
[10] 赵淼,林毅,蔡永萍,等.不同光质对草莓果实成熟过程中色素类物质含量的影响[J] 浙江农业学报,2008,20(1):64-66.
[11] Suzan E H,Robert M S,Davies W J.Photosynthetic activities of vegetative and fruit tissues of tomato[J] Journal of Experimental Botany,1998,48:1173-1181.
[12] Laval-Martin D,Farineau J,Diamond J.Light verus dark carbon metabolism and photosynthetic activity in cherry tomoto fruits I.Occurrence of photosynthesis.Study of intermediates[J] Plant Physiology,1997,60:872-876.
[13] Marcelis L F M,Hofman-Eijer L R B.The contribution of fruit photosynthesis to the carbon requirement of cucumber fruits as affected by irradiance,temperature and ontogeny[J] Physiologia Plantarum,1995,93:476-483.
[14] Steer B T,Pearson C J.Photosynthetic translocation in Capsicum annuun[J] Planta,1976,128:155-162.
[15] Robert M S,Suzan E H,Davies W J.Photosynthetic activity of the calyx,green shoulder,pericarp and locular parenchyma of tomato fruit[J] Journal of Experimental Botany,1999,50:707-718.
[16] Genty B,Briantais J M,Baker N R.The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J] Biochimicaet Biophysica Acta,1989,990:87-92.
[17] Braun G,Malkin S.Regulation of the imbalance in light excitation between photosystemⅡand photosystem I by cations and by the energized state of the thylakoid membrane[J] Biochimcaet Biophysica Acta,1990,1017:79-90.
[18] 储钟稀,童哲,冯丽洁,等.不同光质对黄瓜叶片光合特性的影响[J] 植物学报,1999,41(8):867-870.
[19] Ichiro Terashima,Takashi Fujita,Takeshi Inoue,et al.Green light drives leaf photosynthesis more efficiently than red light in strong white light:Revisiting the enigmatic question of why leaves are green[J] Plant Cell Physiol,2009,50(4):684-697.
[20] 张瑞华,徐坤,董灿兴.光质对生姜叶片光合特性的影响[J] 中国农业科学,2008,41(11):3722-3727.
[21] Demmigadams B,Adams W W,Barker D H,et al.Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation to of excess excitation[J] Physiol Plant,1996,98:253-264.
[22] 贾虎森,李德全,韩亚琴.高等植物光合作用的光抑制研究进展[J] 植物学通报,2000,17(3):218-224.
[23] 康琅,汪良驹.ALA 对西瓜叶片叶绿素荧光光响应曲线的影响[J] 南京农业大学学报,2008,31(1):31-36.
[24] 褚四敏,陈敏洁,贾文姝,等.光质对植物组织培养的影响[J] 安徽农业科学,2011(2):47-48,54.
[25] 吴晓雷,尚春明,张学东,等.番茄品种耐弱光性的综合评价[J] 华北农学报,1997,17(2):98-102.

选择文件类型/文献管理软件名称

选择包含的内容