摘要： 选用烤烟K326包衣品种于2006年进行漂浮育苗，研究了氮素对烟苗蛋白质组分含量、硝酸还原酶活性和干质量积累的影响。试验设3个处理，N0(前期氮素胁迫，后期氮素浓度为100 mg/L)、N1(前后期氮素浓度均为100mg/L)、N2(前后期氮素浓度均为200 mg/L)。结果表明，播种后35～56 d，各处理水溶蛋白含量与总可溶蛋白含量变化趋势完全一致，两者呈极显著相关关系，但水溶蛋白含量仅占总可溶蛋白含量的55.6%～79.6%；各处理醇溶蛋白含量变化呈单峰曲线，峰值出现在播种后49 d；播种后42～56 d，不同处理碱溶蛋白含量表现为：N2>N1>N0。氮素浓度过大反而降低烤烟叶片NR活性，不利于烟苗的氮素代谢；播种后35～56 d，3个处理根干质量均表现为N1>N2>N0，而N1，N2叶干重均大于N0。

关键词: 氮素, 烟苗, 蛋白质组分, 硝酸还原酶, 干质量, 烤烟

Abstract: The effects of nitrogen on the contents of protein components, NR activities and dry weight accumulation in the seedlings of flue-cured tobacco K326 in the floating system were studied in 2006. The experiment involved three treatments: N0: nitrogen was not applied in early stage and 100 mg/L nitrogen was applied in later stage; N1: 100 mg/L nitrogen was applied in both stages; N2: 200 mg/L nitrogen was applied in both stages. The results indicated that during 35-56 days after seedling, the changing tendency of water-soluble protein content for each treatment was the same as that of total soluble protein content, the correlation between which was highly significant. The content of water-soluble protein accounted for 55.6%-79.6% of total soluble proteins. The changes of alcohol-soluble protein content of each treatment presented a single-peak curve and the peak appeared on the 49th day after seedling. The contents of alkali-soluble proteins among the three treatments. Took on the tendency of N2>N1>N0 after seedling 42 d. N2 decreased the NR activities. The root dry weights in the three treatments were in order of N1>N2>N0, while the leaf dry weights of both N1 and N2 were greater than that of N0.

Key words: Nitrogen, Seedlings, Protein components, NR activities, Dry weight, Flue-cured tobacco

中图分类号: 

• S572.01