非乳用山羊乳蛋白结构基因座遗传多态性研究

doi:10.3321/j.issn:1000-7091.2007.01.014

华北农学报 ›› 2007, Vol. 22 ›› Issue (1): 59-63. doi: 10.3321/j.issn:1000-7091.2007.01.014

所属专题： 畜牧；生物技术；

非乳用山羊乳蛋白结构基因座遗传多态性研究

白文林^1,2, 尹荣焕¹, 郑玉才³, 赵素君⁴, 张守纯¹, 王杰³

1. 沈阳农业大学畜牧兽医学院, 辽宁沈阳 110161;
2. 吉林大学畜牧兽医学院, 吉林长春 130062;
3. 西南民族大学生命科学与技术学院, 四川成都 610041;
4. 四川省畜牧科学研究院, 四川成都 610066

收稿日期:2006-11-20 出版日期:2007-02-28
作者简介:白文林(1974-)，男，甘肃永靖人，讲师，博士研究生，主要从事动物遗传育种方面的研究工作。
基金资助:
国家自然科学基金资助项目(39870607)

Genetic Polymorphism of Structural Loci of Milk Protein in Non-dairy Type Goats

BAI Wen-lin^1,2, YIN Rong-huan¹, ZHENG Yu-cai³, ZHAO Su-jun⁴, ZHANG Shou-chun¹, WANG Jie³

1. Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang 110161, China;
2. Animal Science and Veterinary Medicine College, Jilin University, Changchun 130062, China;
3. Life Science and Technology College, Southwest University for Nationalities, Chengdu 610041, China;
4. Animal Science Research Academy of Sichuan Province, Chengdu 610066, China

Received:2006-11-20 Published:2007-02-28

摘要/Abstract

摘要： 为了解非乳用山羊不同群体编码乳蛋白结构基因座的遗传多态性，揭示不同群体间的遗传关系。采用聚丙烯酰胺凝胶电泳(PAGE)技术，分析中国9个非乳用山羊群体共316只个体编码乳蛋白(酶)7个结构基因座的遗传多态性，并基于5个结构基因座等位基因频率计算Nei氏遗传距离，以UPMGA法作聚类分析。研究结果表明，9个山羊群体中，编码乳蛋白(酶)的κ-CN，β-CN，α_s1-CN，α_s2-CN，β-Lg，SA和LDH7个结构基因座在PAGE上均呈现出不同程度的遗传多态性；9个山羊群体中在κ-CN，β-CN，α_s2-CN，β-Lg和SA5个结构基因座上的平均基因一致度、平均基因杂合度和平均有效等位基因数分别为0.3350，0.6552和1.8199.基于κ-CN，β-CN，α_s2-CN，β-Lg和SA5个结构基因座的等位基因频率计算Nei氏遗传距离，以UPMGA法可将中国9个非乳用山羊群体划分为2大类。非乳用山羊群体中，乳蛋白结构基因座是1个遗传多样性相对丰富的系统；乳蛋白结构基因座在非乳用山羊群体划分中具有较强的代表性。

关键词: 非乳用山羊, 乳蛋白, 结构基因座, 遗传多态性, 聚类分析

Abstract: The objective of this experiment was to understand the genetic polymorphism of structural loci encoding milk protein in different Chinese non-dairy type goat populations, to reveal the genetic relationship among those goat populations. Genetic polymorphism of 7 structural loci encoding milk protein (enzyme) of 316 individuals from 9 Chinese non-dairy type goat populations was analyzed by PAGE, and the clustering of these goat populations was researched based on the allele frequency of 5 structural loci encoding milk protein. In 9 goat populations, κ-CN, β-CN, α_s1-CN, α_s2-CN, β-Lg, SAand LDH 7 structural loci encoding milk protein (enzyme) took on different extent genetic polymorphism on PAGE gel. The mean gene homozygosity, mean gene heterozygosity and mean number of effective allele is 0.3350, 0.6552 and 1.8199 based on the gene frequency of κ-CN, β-CN, α_s2-CN, β-Lg and SA respectively. The 9 Chinese goat populations were classified as 2 groups based on the gene frequency of κ-CN, β-CN, α_s2-CN, β-Lg and SA. In 9 Chinese non-dairy type goat populations, the structural loci encoding milk protein were relatively abundant system of genetic diversity; we think that structural loci of encoding milk protein have a relatively strong representation in the classification of non-dairy goat populations.

Key words: Non-dairy goat, Milk protein, Structural locus, Genetic polymorphism, Analysis of clustering

中图分类号:

S827.01

白文林, 尹荣焕, 郑玉才, 赵素君, 张守纯, 王杰. 非乳用山羊乳蛋白结构基因座遗传多态性研究[J]. 华北农学报, 2007, 22(1): 59-63. doi: 10.3321/j.issn:1000-7091.2007.01.014.

BAI Wen-lin, YIN Rong-huan, ZHENG Yu-cai, ZHAO Su-jun, ZHANG Shou-chun, WANG Jie. Genetic Polymorphism of Structural Loci of Milk Protein in Non-dairy Type Goats[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 59-63. doi: 10.3321/j.issn:1000-7091.2007.01.014.

http://www.hbnxb.net/CN/Y2007/V22/I1/59

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