华北农学报 ›› 2014, Vol. 29 ›› Issue (3): 105-108. doi: 10.7668/hbnxb.2014.03.020

所属专题: 小麦 抗旱节水

• 论文 • 上一篇    下一篇

利用IRAP分子标记对小麦抗旱品种筛选的研究

李卫涛1, 陈秋芳1, 押辉远2, 谷运红1   

  1. 1. 郑州大学 河南省离子束生物工程重点实验室, 河南 郑州 450052;
    2. 洛阳师范学院 生命科学系, 河南 洛阳 471022
  • 收稿日期:2014-03-26 出版日期:2014-06-28
  • 通讯作者: 谷运红(1976-),女,河南新密人,副教授,博士,主要从事分子遗传学与生物物理学研究。
  • 作者简介:李卫涛(1987-),男,河南荥阳人,在读硕士,主要从事离子束生物效应和生物物理学研究。
  • 基金资助:
    国家自然科学基金项目(11375154)

Analysis of Screening Wheat Drought Resistance Varieties by IRAP

LI Wei-tao1, CHEN Qiu-fang1, YA Hui-yuan2, GU Yun-hong1   

  1. 1. Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, China;
    2. Institution of Life Science, Luoyang Normal University, Luoyang 471022, China
  • Received:2014-03-26 Published:2014-06-28

摘要: 采用IRAP分子标记方法,设计引物对36份小麦材料进行PCR扩增,得到多态性丰富的指纹图谱,并进行聚类分析。结果显示:扩增出清晰稳定条带246条,片段大小多集中在250~2 000 bp,多态性位点总计13个,各个品种扩增的位点数为0~12个,聚类分析结果能够反映36份小麦材料抗性有无以及强弱关系,表明IRAP分子标记适合应用于小麦抗旱品种筛选的研究分析。

关键词: IRAP分子标记, 小麦, 抗旱性, 聚类分析

Abstract: Using IRAP molecular method, design primers and 36 wheat varieties were used for PCR amplification, polymorphic finger maps were obtained and clustering analysis.The results were as follows:246 bands were produced by IRAP, the range of amplification fragments were 250-2 000 bp, a total of 13 polymorphic loci, the range of different varieties was 0-12.the results of clustering analysis can be clearly differentiate drought resistance between 36 wheat varieties.The study showed that IRAP were suitable for screening drought resistance varieties in wheat.

Key words: IRAP molecular marker, Wheat, Drought resistance, Cluster analysis

中图分类号: 

引用本文

李卫涛, 陈秋芳, 押辉远, 谷运红. 利用IRAP分子标记对小麦抗旱品种筛选的研究[J]. 华北农学报, 2014, 29(3): 105-108. doi: 10.7668/hbnxb.2014.03.020.

LI Wei-tao, CHEN Qiu-fang, YA Hui-yuan, GU Yun-hong. Analysis of Screening Wheat Drought Resistance Varieties by IRAP[J]. journal1, 2014, 29(3): 105-108. doi: 10.7668/hbnxb.2014.03.020.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.2014.03.020

               http://www.hbnxb.net/CN/Y2014/V29/I3/105