华北农学报 ›› 2006, Vol. 21 ›› Issue (6): 45-48. doi: 10.3321/j.issn:1000-7091.2006.06.012

所属专题: 萝卜

• 论文 • 上一篇    下一篇

不同预处理方式对萝卜小孢子活力的影响

付传翠1, 张丽2, 宫国义2, 王富1   

  1. 1. 莱阳农学院,园艺系, 山东青岛 266109;
    2. 北京市农林科学院蔬菜研究中心, 北京 100089
  • 收稿日期:2005-12-12 出版日期:2006-12-28
  • 通讯作者: 张丽
  • 作者简介:付传翠(1980- ),女,山东莱阳人,硕士研究生,主要从事蔬菜花培育种技术的研究。
  • 基金资助:
    北京市科技新星项目(H020821470130);北京市自然科学基金项目(6062011)

Effect of Different Pretreatment on the Vigor of Microspores in Radish(Raphanus sativus L)

FU Chuan-cui1, ZHANG Li2, GONG Guo-yi2, WANG Fu1   

  1. 1. Horticultural Department of Laiyang Agricultural College, Qingdao 266109, China;
    2. Vegetable Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, China
  • Received:2005-12-12 Published:2006-12-28

摘要: 研究温度(低温和高温)及培养基(无碳源、甘露醇及秋水仙素)预处理对4个萝卜基因型小孢子活力的影响,得出的结果是:4℃低温条件下处理3 d的小孢子可保持较高的存活率,36℃高温处理后的小孢子存活率明显低于对照;加入蔗糖的培养基对提高小孢子存活率作用明显;甘露醇及秋水仙素处理也能提高萝卜小孢子存活率,其合适的浓度在不同萝卜基因型中存在差异。

关键词: 萝卜, 预处理, 小孢子活力

Abstract: Effects of low temperature,high temperature,sugar-free,colchicines and mannitol pretreatment were studied on the survival rate of microspores in 4 radish cultivars.The result show that microspores had high survival rate under 4℃ after 3 days treatment,while 36℃ treatment reduced the survival rate.The media with sugar,mannitol and colchicines enhanced the vigor of microspores.And the concentrations of mannitol and colchicines had different effects on 4 radish cultivars.

Key words: Radish, Pretreatment, Survival rate of microspores

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引用本文

付传翠, 张丽, 宫国义, 王富. 不同预处理方式对萝卜小孢子活力的影响[J]. 华北农学报, 2006, 21(6): 45-48. doi: 10.3321/j.issn:1000-7091.2006.06.012.

FU Chuan-cui, ZHANG Li, GONG Guo-yi, WANG Fu. Effect of Different Pretreatment on the Vigor of Microspores in Radish(Raphanus sativus L)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(6): 45-48. doi: 10.3321/j.issn:1000-7091.2006.06.012.

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