华北农学报 ›› 2006, Vol. 21 ›› Issue (5): 91-95. doi: 10.3321/j.issn:1000-7091.2006.05.022

所属专题: 油料作物

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大豆感染灰斑病菌后叶片中多酚氧化酶活性的变化

张丽娟1, 杜金哲1, 杨庆凯2   

  1. 1. 莱阳农学院植物科技学院, 山东青岛 266109;
    2. 东北农业大学大豆研究所, 黑龙江哈尔滨 150030
  • 收稿日期:2006-03-26 出版日期:2006-10-28
  • 作者简介:张丽娟(1969- ),女,博士,副教授,主要从事大豆遗传育种研究工作。
  • 基金资助:
    国家自然科学基金资助项目(39770449)

Study on the Changes of Polyphenol Oxidase in Soybean Varieties Leaves Infected by Cercospora sojina Hara

ZHANG Li-juan1, DU Jin-zhe1, YANG Qing-kai2   

  1. 1. Laiyang Agricultural University, Qingdao 266109, China;
    2. Northeast Agricultural University, Harbin 150030, China
  • Received:2006-03-26 Published:2006-10-28

摘要: 分析了大豆感染灰斑病菌后叶片中多酚氧化酶酶活性的动态变化,探讨了多酚氧化酶在大豆抗灰斑病中的作用。研究的结果表明,抗病品种感染灰斑病菌后,叶片中多酚氧化酶活性均比对照增加;而感病品种接种后叶片中多酚氧化酶活性比对照下降。这表明多酚氧化酶在大豆抗灰斑病中起着重要的作用,其活性的大小与大豆抗病性成正相关,可以作为大豆品种抗性鉴定的一个重要生理生化指标。

关键词: 大豆, 灰斑病菌, 多酚氧化酶

Abstract: The polyphenol oxidase(PPO) activity in leaves of soybean varieties with different resistance to frog-eye leaf spot inoculated with 10 races of Cercospora sojina Hara was measured and the relationship between the changes of PPO activities in leaves and varieties resistance was discussed.The results showed that the changes of PPO activity in resistant varieties leaves increased at various time after inoculated with most of races of Cercospora sojina Hara,but that of susceptible varieties decreased.The PPO activities in leaves of all varieties tested increased.Therefore,the changes of PPO activity in varieties leaves can be a biochemical marker for identification of soybean resistance to frog-eye leaf spot.

Key words: Soybean, Cercospora sojina Hara, Polyphenol oxidase

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引用本文

张丽娟, 杜金哲, 杨庆凯. 大豆感染灰斑病菌后叶片中多酚氧化酶活性的变化[J]. 华北农学报, 2006, 21(5): 91-95. doi: 10.3321/j.issn:1000-7091.2006.05.022.

ZHANG Li-juan, DU Jin-zhe, YANG Qing-kai. Study on the Changes of Polyphenol Oxidase in Soybean Varieties Leaves Infected by Cercospora sojina Hara[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(5): 91-95. doi: 10.3321/j.issn:1000-7091.2006.05.022.

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