内标18S rRNA和-β<em>actin</em> mRNA比较：肉鸡十二指肠SGLT1 mRNA的发育规律

doi:10.3321/j.issn:1000-7091.2006.03.026

华北农学报 ›› 2006, Vol. 21 ›› Issue (3): 112-116. doi: 10.3321/j.issn:1000-7091.2006.03.026

所属专题： 畜牧；

内标18S rRNA和-βactin mRNA比较：肉鸡十二指肠SGLT1 mRNA的发育规律

王修启, 谭会泽, 苏海林, 宋予震, 冯定远

华南农业大学动物科学学院,广东,广州,510642

收稿日期:2005-12-24 出版日期:2006-06-28
通讯作者: 冯定
作者简介:王修启（1968- ），男，河南新乡人，在站博士后，副研究员，主要从事动物分子营养学研究。
基金资助:
国家重点基础研究发展计划（973）项目（2004CB117501）；广东省自然科学基金项目（05300836）

Comparison Between 18S rRNA and β-actin mRNA as Internal Standard: Ontogenetic Expression of SGLT1 mRNA in Duodenum of Broiler Chicken

WANG Xiu-qi, TAN Hui-ze, SU Hai-lin, SONG Yu-zhen, FENG Ding-yuan

College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received:2005-12-24 Published:2006-06-28

摘要/Abstract

摘要： β-actin mRNA和18S rRNA因其在相同试验条件下的稳定表达，而被广泛用作内标基因来确定目的基因的表达量。试验分别使用这2种内标基因，用相对定量RT-O36d）十二指肠钠葡萄糖共转运载体1（Sodium glucose cotransporter 1，SGLT1）的发育规律进行了分析研究，比较不同内标基因对SGLT1 mRNA表达水平检测结果的影响，以确定两者在一致试验条件下表达的相似度。结果表明，分别以-βactinmRNA和18SrRNA作为内标，SGLT1 mRNA表达发育规律完全一致，均表现为2~30d不断升高，44d下降，58d回升；2d和44 d的SGLT1 mRNA丰度显著低于16d（P<0.05）和30d（P<0.05），58d的SGLT1 mRNA表达水平显著低于30d（P<0.05）。提示这2种管家基因均可以作为内标用于肉鸡肠道转运载体基因mRNA发育规律的相对定量研究。

关键词: 18SrRNA, -&beta, actinmRNA, 内标, SGLT1mRNA, 肉鸡

Abstract: Beta-actin mRNA and 18S rRNA are widely employed as internal control genes,with the assumption that they are expressed constitutively to a similar degree under same experimental conditions.The objective of this study was to investigate the SGLT1(Sodium glucose cotransporter 1) mRNA ontogenetic expression in duodenum of broiler chicken(0-58 d) by a semi-quantitative RT-PCR,using beta-actin mRNA and 18S rRNA as internal standard respectively.In this study,we tested this assumption by assessment of the transcription of these two genes in chicken intestinal tissues using a semi-quantitative RT-PCR.The results indicate showed that the SGLT1 mRNA ontogenetic expression were consistent whether beta-actin mRNA or 18S rRNA was acted as internal control.The SGLT1 mRNA abundance in duodenum of chicken increased from day 2 to day 30,then started to decline from day 44,and increased again from day 58.The SGLT1 mRNA expression level on day 2 and day 44 were lower significantly than that on day 16(P<0.05) and day 30(P<0.05) respectively.And the SGLT1 mRNA abundance on day 58 was lower significantly than that on day 30(P<0.05).It suggested that both beta-actin mRNA and 18S rRNA could be used as internal standard for semi-quantitative analysis of transport carrier gene mRNA in intestinal ontogenesis of broiler chicken.

Key words: 18S rRNA, β-actinactin mRNA, Internal standard, SGLT1 mRNA, Broiler chicken

中图分类号:

S831

王修启, 谭会泽, 苏海林, 宋予震, 冯定远. 内标18S rRNA和-βactin mRNA比较：肉鸡十二指肠SGLT1 mRNA的发育规律[J]. 华北农学报, 2006, 21(3): 112-116. doi: 10.3321/j.issn:1000-7091.2006.03.026.

WANG Xiu-qi, TAN Hui-ze, SU Hai-lin, SONG Yu-zhen, FENG Ding-yuan. Comparison Between 18S rRNA and β-actin mRNA as Internal Standard: Ontogenetic Expression of SGLT1 mRNA in Duodenum of Broiler Chicken[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 112-116. doi: 10.3321/j.issn:1000-7091.2006.03.026.

http://www.hbnxb.net/CN/Y2006/V21/I3/112

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内标18S rRNA和-βactin mRNA比较：肉鸡十二指肠SGLT1 mRNA的发育规律

Comparison Between 18S rRNA and β-actin mRNA as Internal Standard: Ontogenetic Expression of SGLT1 mRNA in Duodenum of Broiler Chicken

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内标18S rRNA和-βactin mRNA比较：肉鸡十二指肠SGLT1 mRNA的发育规律

Comparison Between 18S rRNA and β-actin mRNA as Internal Standard: Ontogenetic Expression of SGLT1 mRNA in Duodenum of Broiler Chicken

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