摘要： 参照NDVLa Sota株核酸序列（AF077761）设计1对引物，利用RT-PCR扩增F基因并得到了长为1700bp的片段，将其克隆到pGEM-T easy vector中，经酶切鉴定和测序后克隆进原核表达载体PET_32a，将重组表达质粒转化BL21（DE3），在IPTG诱导下表达约83kd的融合蛋白，SDS_PAGE电泳和Western blotting检测证实该基因片段获得高效表达且表达产物具有免疫学活性。

关键词: 新城疫病毒, F基因, 克隆, 原核表达

Abstract: A pair of primers was designed according to the sequence of F gene of NDV La Sotastrain(AF077761) for amplifying the F gene by RT-PCR.1 700 bp DNA fragment was amplified and cloned into pGEM-T easy vector.After restriction endonuclease analysis and sequencing,the fragment was inserted into the expression vector PET_32a.The recombinant plasmid produced a 83 kd fusion protein in E.coli BL21(DE3) under the induction of IPTG.The expression quantity and immunoreactivity of the fusion protein was detected by SDS_PAGE electrophoresis and Western blotting.

Key words: Newcastal disease virse (NDV), F gene, Clone, Prokaryotic expression

中图分类号: 

• S831.5