华北农学报 ›› 2006, Vol. 21 ›› Issue (2): 13-17. doi: 10.3321/j.issn:1000-7091.2006.02.004

所属专题: 番茄

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番茄红素快速测定方法的研究

刘仲齐, 薛俊, 金凤媚, 郏艳红   

  1. 天津市农业生物技术研究中心, 天津 300384
  • 收稿日期:2005-10-09 出版日期:2006-04-28
  • 作者简介:刘仲齐(1964- ),男,甘肃清水人,研究员,博士,主要从事番茄分子育种研究工作。
  • 基金资助:
    天津市应用基础重点项目(033803611)

Study on Method for Fast Measuring Lycopene Content in Tomato

LIU Zhong-qi, XUE Jun, JIN Feng-mei, JIA Yan-hong   

  1. Tianjin Research Center of Agricultural Bio-technology, Tianjin 300384, China
  • Received:2005-10-09 Published:2006-04-28

摘要: 以番茄红素含量不同的8个基因型为材料,对CR400型色差计的快速测定值与番茄红素含量之间的相关性进行了研究。结果表明,以果实为样品测定的L1,a1,b1值在基因型间呈随机分布,番茄红素含量与以果浆为样品测定的L2和b2值呈负相关,与a2值呈极显著正相关。以去除自由水后的果酱为样品测定的L3,a3,b3值更加稳定,它们与番茄红素含量的相关性更加密切。自由水含量对色差计的读数值影响较大,在自由水含量适中(71.11%~76.20%)的基因型中,番茄红素含量与(a3/b3+a3/L3)的相关系数高达0.808**,此时用线性回归方程能够比较准确地根据色差计的读数值估测果实中番茄红素的含量。

关键词: 番茄, 番茄红素, 测定方法

Abstract: Fifty-eight tomato genotypes with different lycopene content were used to study the relationship between their lycopene content and color readings of CR400.Experimental results showed that L1,a1 and b1 from fruits were randomly distributed among genotypes.But lycopene content was negatively correlated with L2 and b2 of fruit puree respectively and positively correlated with a2.After free water in fruit puree was discarded by centrifugation,readings of L3,a3 and b3 from these pastes were more stable and displayed a more precise linear relationship to lycopene content.Free water imposed a significant effect on color readings.Among genotypes with free water content between 71.11% and 76.20%,the correlation coefficient between lycopene content and (a3/b3+a3/L3) was as high as 0.808.Therefore,linear regression equation could be used to predict lycopene content of fruits based on their color readings from paste without free water.

Key words: Tomato, Lycopene, Measure method

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引用本文

刘仲齐, 薛俊, 金凤媚, 郏艳红. 番茄红素快速测定方法的研究[J]. 华北农学报, 2006, 21(2): 13-17. doi: 10.3321/j.issn:1000-7091.2006.02.004.

LIU Zhong-qi, XUE Jun, JIN Feng-mei, JIA Yan-hong. Study on Method for Fast Measuring Lycopene Content in Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 13-17. doi: 10.3321/j.issn:1000-7091.2006.02.004.

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