华北农学报 ›› 2004, Vol. 19 ›› Issue (4): 1-3. doi: 10.3321/j.issn:1000-7091.2004.04.001

所属专题: 甜瓜 生物技术

• 论文 •    下一篇

分子标记在辅助甜瓜“A”基因转育上的研究

李秀秀, 吕敬刚, 彭冬秀, 赵宗武   

  1. 天津科润蔬菜研究所, 天津 300384
  • 收稿日期:2004-01-09 出版日期:2004-12-28
  • 作者简介:李秀秀(1954-),女,河北行唐人,研究员,硕士,主要从事甜瓜育种研究工作.
  • 基金资助:
    天津市自然科学基金资助项目(013802411);国家863项目(2002AA244011-3)

Primary Report on Research of Marker in Helping Gene A Transport of Melon

LI Xiu-xiu, L Jing-gang, PENG Dong-xiu, ZHAO Zong-wu   

  1. Tianjin Kernel Vegetable Research Institute, Tianjin300384, China
  • Received:2004-01-09 Published:2004-12-28

摘要: 对甜瓜雄花两性花同株与雌雄异花同株材料间杂交后代及回交后代的花性型分离研究表明,F2群体中单性花性状呈现单显性基因控制的3∶1分离比例,证明供试材料雄花两性花同株与雌雄异花同株的基因型分别为aaGG和AAGG。并运用RAPD技术,在F2群体中采用混合分组分析法对A基因进行了分子标记筛选,找到一个与A基因连锁的大小约为500bp的RAPD标记。

关键词: 甜瓜, 雌雄异花同株, RAPD, 基因型

Abstract: Segregation patterns of flower types were analyzed in F2 and BC1 populations from each of two crosses between monoecious and andromonoecious materials of melon ( Cucumis melo). Results showed that flower type difference between the two types of parents was controlled by a pair of genes at one single locus a, with AAGG and aaGG genotypes, respectively, as flower type segregation in F2s and BC1s from the two crosses was in agreement with simple Mendelian ratios of 3B1 and 1B1, respectively. RAPD primers were screened for polymorphisms between monoecious and andromonoecious gene pools from F2of Mr @ YF cross using bulked seg- regant analysis ( BSA) strategy. One polymorphic fragment ( about 500 bp) was amplified, which will hopefully serve as a marker in marker-assisted selection in a backcross breeding programme for monoecious flower type.

Key words: Cucumis melo, Monoecious plant, RAPD, Genetype

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引用本文

李秀秀, 吕敬刚, 彭冬秀, 赵宗武. 分子标记在辅助甜瓜“A”基因转育上的研究[J]. 华北农学报, 2004, 19(4): 1-3. doi: 10.3321/j.issn:1000-7091.2004.04.001.

LI Xiu-xiu, L Jing-gang, PENG Dong-xiu, ZHAO Zong-wu. Primary Report on Research of Marker in Helping Gene A Transport of Melon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(4): 1-3. doi: 10.3321/j.issn:1000-7091.2004.04.001.

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