摘要： 对盐、旱逆境下转BADH基因小麦品系99T6的生长发育、甜菜碱醛脱氢酶活性及甜菜碱积累等进行了研究分析,结果表明,转BADH基因株系在盐逆境下具有明显的生长优势,耐盐能力达到100mmol/L;电导率和质膜相对透性表明转基因株系抗膜损伤能力增强;甜菜碱醛脱氢酶活性的增强和甜菜碱积累的增加,说明转基因株系的盐旱逆境抗性是通过甜菜碱积累这一长期、持久的方式解除渗透胁迫,而不是通过脯氨酸积累这种临时的应急反应;以甜菜碱作为靶标性状采用基因工程方法提高植物盐旱耐性是可行的。

关键词: 小麦, 转基因, 甜菜碱醛脱氢酶活性, 耐盐性

Abstract: Assay for salt tolerance and drought resistance of t ransgenic wheat with BADH cDNA by biolist icmethod were conducted under simulated salt/drought stress condition. The results indicated that the t ransgenicwheat has many obvious advantages over its received plant s,such as the more vigorous development of seedlings,the well developed root system and the greater root act ivity under salt/ drought stress condition,as well as theimproved plasma membrane protection of ex cised leaf. The betaine aldehyde dehydrogenase enzyme activity underdif ferent salt concentration is also hig her,and more betaine accumulation under drought condition,than it sreceived plant. This indicated that the int roduction of BADH gene into wheat may affect a series of physiolog icalreaction to adapt st ress condition,this may be one of the reasons for its higher salt/drought tolerance. The w ayto increase crop stress tolerance/ resistance through transgenic method may be effect ive.

Key words: Transgenic, Transgenic w heat, BADH, Drought resistance, Salt tolerance

中图分类号: 

• S512.01