华北农学报

所属专题: 小麦 盐碱胁迫 生物技术

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外源甜菜碱醛脱氢酶(BADH)基因对小麦合成甜菜碱的影响

丁占生1, 张艳敏1, 张光荣2, 温之雨1, 郭北海1   

  1. 1. 河北省农林科学院遗传生理研究所,石家庄,050011;
    2. 涿鹿县农业技术推广中心,涿鹿县,075600
  • 收稿日期:2003-06-20 出版日期:2003-12-31
  • 通讯作者: 郭北海
  • 作者简介:丁占生(1971- ),男,河北定州人,硕士,助理研究员,主要从事植物生物技术研究工作。
  • 基金资助:
    国家863计划(2001AA212121-3);植物转因及其产业化专项资助课题(J99-B-010)。

The Effect of Foreign Betaine Aldehyde Dehydrogenase Gene on the Synthesis of Betaine in Wheat

DING Zhan-sheng1, ZHANG Yan-min1, ZHANG Guang-rong2, WEN Zhi-yu1, GUO Bei-hai1   

  1. 1. Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Science,Shijiazhuang 050051,China;
    2. Zhuolu Extension Center of Agricultural Technology,Zhuolu 075600,China
  • Received:2003-06-20 Published:2003-12-31

摘要: 通过毛细管电泳,对转入山菠菜BADH基因的小麦品系T1,T4,T6及受体石4185的甜菜碱含量进行了检测,发现在胁迫与未胁迫条件下,T1,T4,T6的甜菜碱含量均高于受体石4185,T1,T6与受体差异显著,T4与受体差异不显著。说明外源BADH基因可提高小麦中甜菜碱含量,但在不同品系中的表达有一定差异。

关键词: BADH, 甜菜碱, 毛细管电泳, 小麦

Abstract: By capillary electrophoresis,the betaine content of T1,T4,T6 show ed with foreign BADH f romAt riplex hortenis,and the accepted wheat of shi 4185,was deter mined.The results that the betaine content of T1,T4,T6 was hig her than shi 4185,and the dif ference between T1,T6 and shi 4185,except T4 and shi4185,was sig nificant.It was indicated that the foreign BADH gene could improve the betaine co ntent in wheat,but the improvement s were not the same in different stains.

Key words: BADH, Betaine, Capillary elect rophoresis, Wheat

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引用本文

DING Zhan-sheng, ZHANG Yan-min, ZHANG Guang-rong, WEN Zhi-yu, GUO Bei-hai. The Effect of Foreign Betaine Aldehyde Dehydrogenase Gene on the Synthesis of Betaine in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.010.

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