摘要： 以含对叶霉病不同抗性基因的番茄品种为材料,研究了叶霉病菌侵染过程中PAL酶活性和次生代谢物木质素含量的变化与品种抗性、病原菌生理小种间的相互关系。接种后苯丙氨酸解氨酶活性增加,抗病品种的PAL酶活性高峰出现早于感病品种。番茄品种的抗病性与其接种后36hPAL酶活性变化百分比呈极显著正相关(r=0.96)。另外,还成功地利用PAL酶活性变化速度这一指标对叶霉病生理小种的变异进行了生化鉴定。

关键词: 番茄, 叶霉病, 苯丙氨酸解氨酶, 抗病指标

Abstract: Several varieties of tomato with di fferent degrees of resistance to cladosporium fulvum were used to study the changes in phenylalanine ammonia lyase(PAL) activi ty and lignin conten as well as their correlationship with the resistance degree of varie t ies and pathogeny during inoculation.PAL activity inc reased through inoculation.PAL peaks peared earl ier in resistant varieties than those in susceptible ones.There was signi ficant variet ies correlation between the seedling resistance and the increase of PAL ac t ivi ty during 36 hours after inoculation(r= 0.96).The changing speed of PAL activi ty as a resis tance inde x was applied successfully in identi fying the di fferentiation in physiological varieties of Cladosporium fulvum.

Key words: Tomato, Cladosporium fulvum, PAL, Resistance index

中图分类号: 

• S436.412