摘要： 对怀山药带芽茎段和无芽茎段的离体培养研究结果表明：（1）在相同的培养条件下，无芽茎段有利于愈伤组织的形成，但不同的激素及其组合对愈伤组织的诱导效果不同，最佳的激素组合为MS+6-BA1mg/L（单位下同）+NAA0.5；带芽茎段有利于多芽体的形成，在6-BA1~2+NAA0.1的培养茎上，均能形成3~5芽的多芽体，在此基础上，PP₃₃₃能够增加多芽体中芽的数目，而GA则使多芽体中芽的数目减少。（2）通过带芽茎段形成多芽体的方法可以在短时间内繁殖出大量的试管苗，为怀山药优良品种的迅速推广种植提供了一条较为实用的途径。

关键词: 怀山药, 茎段, 愈伤组织, 多芽体

Abstract: The studies on in vitro culture of stem segments with bud and budless segments of Dioscorea opposita showed： (1) In the same culture condition, the budless stem segments were more advantageous to callus formation, but different phytohormones and their combinationhad different effects on callus induction and the most suitable combination were MS+6-BA 1 mg/L+NAA 0.5 mg/L. The stem segments with bud were more favourable to multi-bud body. Multi-bud body with 3-5 buds were formed on MS basic medium containing 6-BA 1-2 mg/L+NAA 0.1 mg/L.On this basis, the number of buds in multi-bud body was increased by adding PP₃₃₃, but reduced by adding GA, to the medium. (2) A large number of plantlets might be reproduced within a short time by way of the formation of multi-bud body by stem segment with bud, thus, providing a useful way for the rapid popularization and planting of excellent varieties of Dioscorea opposita.

Key words: Dioscorea opposita, Stem segment, Callus, Multi-bud body

中图分类号: 

• S632.1043