摘要： 以普通结瘤大豆Braggcv.[Glycinemax（L.）Merr.]及其超结瘤突变体nts382为试验材料，用营养液培养方法研究了缺硼对大豆植株生长和根瘤固氮作用的影响。结果表明，在缺硼条件下，植株根系生长受到抑制，变粗变短，颜色加深；地上部生长点坏死，使植株长得矮小，干重降低；根系结瘤能力和根瘤固氮活性明显下降，说明硼不仅对维持植株的正常生长，同时对根瘤的形成及其固氮活性都起着重要作用。

关键词: 大豆, 缺硼, 固氮酶活性, 根瘤, 超结瘤突变体

Abstract: Effects of boron-deficiency on the growth of soybean [Glycine max (L.) Merr.] Bragg cv., its supernodulating mutant nts 382 plants, and also on nitrogenase activity of nodules were studied using solution culture method. The plants grown in B-free medium showed B-deficiency symptoms first in roots, and then in shoots, including a decrease in the height and weight of the shoots and a decrease in the length and dry weight of roots. The content of B in roots and shoots decreased markedly. Also B content in nodules of the B-deficient plants decreased. Under B-deficiency sttress, the growth of nodules was inhibited, therefore their nitrogenase activity reduced. The results indicated that B was required for the growth and development of plants and nodules, and was important for the nitrogen fixation of the nodules.

Key words: Boron-deficiency, Nitrogenase activity, Soybean [Glycine max (L.) Merr.], Nodule, Supernodulating mutant