摘要： 本试验以中蔬5号番茄种子为材料，研究不同老化程度下，种子萌发初期的CAT活性与种子活力的关系。试验结果表明，活力指数与种子内CAT活性显着相关，r=0.995.同时，CAT标准液催化反应速度曲线显示，在有充足的H2O2时，酶活性与O2产量（记录笔走纸高度）之间有极好的线性关系，r=0.997.因此，测定种子萌发初期的CAT活性，是间接评判种子活力的一种可靠方法。
关键词:
番茄种子,
过氧化氢酶,
种子活力
Abstract: Seeds of tomato(cv.zhongshu.5) were aged in sealed aluminum foil bags under 58±1 oC for 0,6,8,12 days, The germination rate(GR),vigor index(VI),mobilization efficiency(ME), electro conductivity(EC) and catalase(CAT) activity of the unaged and aged seeds were tested. Results show that as the seed ages,the declination of CAT activity is positively related to the decrease of VI, and negatively related to the increase of EC. From these results, it is concluded that CAT activity is a reliable parameter for seed vigor tests, and the optimal duration of reaction lasts 1—2 min.
Key words:
Tomato seed,
Catalase,
Seed vigor
中图分类号:
谷建田, 范双喜, 宋学锋, 欧阳新星. 番茄种子衰老过程中过氧化氢酶(CAT)活性变化[J]. 华北农学报, 1998, 13(2): 112-116. doi: 10.3321/j.issn:1000-7091.1998.02.021.
Gu Jiantian， Fan Shuangxi， Song Xuefeng. Studies on Catalase Activity in Aging of Tomato Seeds[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(2): 112-116. doi: 10.3321/j.issn:1000-7091.1998.02.021.