摘要： 以4种经过人工老化处理不同类型(单秆型白芝麻、分枝型白芝麻、单秆型黑麻和分枝型黑芝麻)芝麻种子为试验材料,采用标准发芽试验、电导率测定、MDA含量测定、POD活性测定和TTC还原量等方法进行种子活力测定,研究老化处理对芝麻种子生理生化的影响,并与模拟田间出苗率进行相关性分析,以确定不同类型芝麻种子活力检测的适宜方法。结果表明:芝麻种子老化过程中,发芽率、发芽势、发芽指数、活力指数、TTC还原量和POD活性逐渐下降,MDA含量逐渐增加,电导率变化趋势材料间不一致;不同种质的抗老化能力存在差异。单秆型白芝麻种子活力检测的适宜方法为发芽率和电导率测定;分枝型白芝麻种子活力检测的适宜方法是发芽率、电导率测定、MDA含量测定、POD活性测定和TTC还原量测定;单秆型黑芝麻种子活力检测的适宜方法是发芽率和POD活性测定;分枝型黑芝麻种子活力检测的适宜方法是发芽率和TTC还原量测定。芝麻在42～43℃、100%RH条件下,人工老化处理的最佳时间为72 h。
关键词:
芝麻,
人工老化,
种子活力,
生理特性,
活力检测方法
Abstract: Four different types of sesame germplasm resources ( Single-stem white sesame,Branched-stem white sesame,Single-stem Black sesame and Branched-stem black sesame ) treated with artificial ageing were used in this study. The standard germination test, electrical conductivity test, MDA content test, POD activity test and TTC re-ductive amounts test were conducted. The result showed that the germination potential,germination rate,germination index,vigor index,TTC reductive amounts and POD activity declined gradually with the increase of seed aging time, whereas MDA content increased gradually. The trend of conductivity change was not consistent between materials. The differences of resistance to artifical aging existed among the four accessions. With the correlation analysis be-tween the seed vigor indexes and the simulated field emergence rate,we got the conclusions that the suitable seed vigor testing methods for Single-stem white sesame were germination rate and electrical conductivity tests;for Branched-stem white sesame were germination rate,electrical conductivity test,MDA content test,POD activity test and TTC reductive amounts test;for Single-stem black sesame were germination rate and POD activity test;and for the Branched-stem black sesame were germination rate and TTC reductive amounts test. The results showed that ac-celerated aging under conditions of 42-43 ℃at 100%RH for 72 h were appropriate for an accelerated aging test for seed vigor in sesame.
Key words:
Sesame,
Accelerated aging,
Seed vigor,
Physiological characteristics,
Vigor testing methords
中图分类号:
梅鸿献, 刘艳阳, 武轲, 杜振伟, 郑永战. 芝麻种子老化处理生理特性变化及种子活力适宜检测方法研究[J]. 华北农学报, 2013, 28(5): 169-174. doi: 10.7668/hbnxb.2013.05.030.
MEI Hong-xian, LIU Yan-yang, WU Ke, DU Zhen-wei, ZHENG Yong-zhan. Study on the Changes of Physiological Characteristics by Artificial Aging and the Suitable Seed Vigor Testing Methods for Sesame[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 169-174. doi: 10.7668/hbnxb.2013.05.030.