摘要： 通过对两种生菜及其不同外植体在培养基上不定芽发生能力的筛选，得到“大湖366”叶切片不定芽发生率高达87%的培养体系，并且丛生状不定芽可陆续发生。将叶切片与农杆菌共培养，对经km筛选后所得再生株进行X-Glu染色，兰色反应阳性率为50%。

关键词: 生菜, 再生, 遗传转化, 农杆菌

Abstract: An efficient procedure for obtaining transgenic lettuce plants using agrobacterium is described. The young leaf sections,when cultured on MS medium with 0.5mg/L BA,0.1 mg/L NAA,had a 87% plant regeneration rate, and more than one shoot were regenerated per regenerable explant during culturing. After co-cultured will a}muacteria,the explants had a 65% regeneration rate on MS medium with 100mg/L kanamycin. These regenerated plants showed a 50% positive reaction frequency when their leaf slices incubated with X-Glu solution.

Key words: Lettuce, Regeneration, Transformation, Agrobacterium

中图分类号: 

• S636.201