摘要： 从小麦CK89、102、113的花药和花冬的约穗诱导愈伤组织的继代培养中，选择致密颗粒型愈伤组织，转入附加2,4-D2～4mg/L改良MS固体培养基上进行继代培养，6～10周后形成生长迅速的胚性愈伤组织，这种愈伤组织分离原生质体得率为1～3.7×10⁷个/g.原生质体于PCM₂培养基上琼脂糖包埋培养，3～5天出现第一次细胞分裂，7～14天进行第二、第三次细胞分裂，两周后形成大量细胞团，细胞分裂频率达20.3%～73.3%,4～6周后形成肉眼可见愈伤组织。供试4个基因型中，CK89、102和花冬均形成再生愈伤组织，仅113形成多细胞团。愈伤组织增殖分化实验尚在进行中。

关键词: 小麦, 胚性愈伤组织, 原生质体培养

Abstract: The compact and granular calli were selected from anther-induced calli of wheat varieties CK89, 102,113 and immature inflorescence-induced calli of Huadong, respectively.While these calli were cultured in the modified MS medium with 2-4mg/L 2,4-D,a friable fast-growing type of embryogenic calli was obtained after 6 to 10 weeks. The high yield protoplasts were isolated from the culture and the protolast yield was 1 to 3.7 × 10'/g calli.The isolated protoplasts were cultuerd in PCMZ medium solidified with 0.4% agarose. The first division occured 3-5 days after plating .The second and third divisions were identifiable during 7 to 14 days. A large number of cell aggregates emerged after two weeks culture, and the division frequency was 20.3%-73.3%. After 4-6 weeks plating, the microcalli formed.The calli were obtained from wheat varieties CK89, 102 and Huadong, and cell aggregates were obtained from 113. An experiment on calli differentiation is under way. A few factors offecting the protoplast cultures were also studied.

Key words: Wheat, Embryogenic calli, Protoplast culture, Protoplast-derived calli

中图分类号: 

• S512.101