摘要： 本文旨在探索用叶片中酸性磷酸酶活性(APA)进行番茄磷素诊断.研究发现:叶饼法(6叶饼或12叶饼)三个反应时问(15、30和60分钟)所测出的功能叶片的APA与叶片的磷含量(P%)、全株(不包括根系)的P含量(P%)及植株干重都呈极显著负相关(P<0.01).匀浆法只有15分钟所测定的功能叶片APA与它们呈极显著负相关(P<0.01).此法比起测定植株体内磷的含量要快速简单得多,平均每15分钟测定一个样,很适用于生产实践.但由于APA不但受P营养状况、株龄及环境因子的影响,而且受测定条件的控制,要建立一个定量的实用的APA指标还相当艰难.
关键词:
番茄,
酸性磷酸酶,
叶饼法,
匀浆法
Abstract: Leaf acid phosphatase activity(APA) is used to diagnose P deficiency in tomato leaves. Results showed that there were significant negative correlations (P<0.01) between the enzyme activity tested with the leaf disc methods and the P concentration in the leaves or whole plants or the dry weight of tomato. Very significant negative correlation (P<0.01) was found in the 15 minutes reaction time with the homogenised leaves method between the enzyme activity and the other 3 elements mentioned above. The assay of the enzyme either with the leaf disc method or with the homogenised leaves method is simple and rapid. Therefore, it can be used to detect P deficiency in the tomato plant. APA is not only affected by P status, plant age and environment, but also by PNPP, pH, temperature and reaction time. So there are many problems to be solved before setting up a critical value for the enzyme activity.
Key words:
Tomato,
Acid phosphatase,
Leaf disc method,
Homogenised leaves method
林启美, 黄德明. 应用酸性磷酸酶进行番茄磷素诊断[J]. 华北农学报, 1991, 6(2): 78-83. doi: 10.3321/j.issn:1000-7091.1991.02.013.
Lin Qimei, Huang Deming. Evaluation of an Acid Phosphatase Assay for Detection of Phosphorus Deficiency in Tomato Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(2): 78-83. doi: 10.3321/j.issn:1000-7091.1991.02.013.