华北农学报 ›› 1989, Vol. 4 ›› Issue (S1): 82-85. doi: 10.3321/j.issn:1000-7091.1989.z1.014

所属专题: 小麦

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普通小麦线粒体的制备及其蛋白质SDS—PAGE分析

司智海, 刘植义   

  1. 河北师范大学生物系, 石家庄
  • 出版日期:1989-12-31

Preparation of Mitochondria from Wheat (T.Aestivum L.) and Analysis of Its Protein with SDS-PAGE

Si Zhihai, Liu Zhiyi   

  1. Department of Biology, Hebei Teachers' University, Shijiazhuang
  • Published:1989-12-31

摘要: 用差速离心和密度梯度离心技术,分离、纯化了普通小麦黄化苗线粒体;以SDS—PAGE分析了多肽构成.实验结果表明:普通小麦线粒体密度梯度离心行为与其它植物相同;在电泳图谱中其多肽呈现40—50条带纹,分子量介于14KD—100KD之间.

关键词: 普通小麦, 线粒体, 密度梯度离心, SDS-PAGE

Abstract: Mitochondria of, wheat (T. aestivum L. ) was prepared and purified through differential centrifugation and density gradient centrifugation. Also,the polypeptide structure of it was analyzed with SDS-PAGE. The results indicated that the density-gradient-behavior of-mitochondria from wheat was the same as other plants',40-50 bands of polypeptide could be seen on the electropheretogram, and their molecular weight were 14KD-100KD.

Key words: Wheat (T. aestivum L.), Mitochondria, SDS-PAGE, Density gradient ceritrifugation

引用本文

司智海, 刘植义. 普通小麦线粒体的制备及其蛋白质SDS—PAGE分析[J]. 华北农学报, 1989, 4(S1): 82-85. doi: 10.3321/j.issn:1000-7091.1989.z1.014.

Si Zhihai, Liu Zhiyi. Preparation of Mitochondria from Wheat (T.Aestivum L.) and Analysis of Its Protein with SDS-PAGE[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1989, 4(S1): 82-85. doi: 10.3321/j.issn:1000-7091.1989.z1.014.

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