摘要： 在培养基中加入一定浓度NaCl，对小麦F₁花药愈伤组织的耐盐变异体，进行细胞水平上的筛选。采用直接筛选法及逐渐增加盐浓度筛选法，结果表明，后者是提高耐盐变异体频率的有效措施。C₁₇+0.1% NaCl（转移愈伤组织）C₁₇+0.3% NaCl及C₁₇+0.1% NaCl（转移愈伤组织）C₁₇+0.5% NaCl处理的绿苗分化率，较直接筛选法分别提高5.06及15.0倍。在较高（加0.3% NaCl）盐含量的培养基上分化的花粉植株及其后代（H₂、H₃）表现耐盐性较强。获得了在0.4%含盐量土壤上种植能正常或基本正常生长的高耐盐株系3个。

关键词: 小麦, 花药愈伤组织, 耐盐变异体

Abstract: By use of anther culture technique,salt-tolcrantc variants of winter wheat were screened at the cell level by adding quantitative NaCl into the medium C₁₇.About 143331 anther of F1 were cultured on the medium by direct screening and incrcasing salinity screening method.The results proved that the later was a more effective measure for obtaining salt tolerante variants.Green differentiation frequencies on C₁₇+0.1%,NaCl-(callus)-C₁₇+0.3% NaCl and C₁₇+0.1 % NaCl-callus-C₁₇+0.5% NaCl increased 5.06 and 15.0 times respectively,compared with direct screening method,by calculating the number of anthers cultured.Pollen plants differentiated on higher salinity medium C₁₇(added 0.3% and 0.5% NaCl)and their progeny(H2 H1)have higher salt-tolerance.They could develop normally or basically normally in the soil containing 0.4% salimity.

Key words: Wheat, Anther callus, Salt-tolerant variant