摘要： 桑冬芽经-3℃--20℃预冻，不加任何保护剂，采用快冻法浸于液氮中（-196℃），取出化冻后，用组织培养法检测，存活率可达55-90%。较好的化冻方法为0-2℃空气解冻。在液氮保存1天与40天的冬芽存活率无差别。经组织培养均生长良好并分化成苗。此结果为桑冬芽长期保存提供了可能性。

关键词: 种质保存, 组织培养, 桑树

Abstract: The winter buds of mulberry were precooled at-3-20℃ and then by the rapid-frreezing method put into liquid nitrogen(LN,-196℃)without the use of a cryoprotectant.The winter buds were taken out from LN arid were thawed.To check the survival rate of winter bud the tissue culture method was used.The result was that survival rate was 55-90%.A better thawing method is 0-2℃ air slow-thawing.After storage for 1 to 40 days in LN,there is no difference in winter bud’s,survival rate.Buds could be normally differentiated arid developed through tissue culture.The results offer possibility for long duration preservation of winter mulberry bud.

Key words: Germplasm cryopresermation, Tissue culture, Mulberry