华北农学报 ›› 1986, Vol. 1 ›› Issue (2): 64-69. doi: 10.3321/j.issn:1000-7091.1986.02.010

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紫外线诱变对联合固氮菌株特性的影响

周枫1, 张启行1, 蒋有绎1, 张美庆1, 刘建玲2   

  1. 1 北京市农林科学院土肥所;
    2 河北农业大学
  • 出版日期:1986-06-28

THE EFFECT OF U V MUTAGEN ON PHYSIOLOGICAL PROPERTIES OF ASSOCIATIVE N2-FIXING BACTERIA

Zhou Feng1, Zhang Qihang1, Jiang Youyi1, Zhang Maiqing1, Liu Jianling2   

  1. 1 Institute of Soil and Fertilizer Research, Beijing Municipal Academy of Agricultural and Forestory Sciences;
    2 Hebei Agricultural University
  • Published:1986-06-28

摘要: 本文报道了用紫外线诱变的方法处理菌株(K.pneumoniae)549,经两次诱变处理,分别筛选到一株具有抗Km400γ的菌株M-1-5-5和一株固氮活性较高的菌株M-1.Km对549和M-1的最低抑菌浓度随着涂皿浓度的变化而改变,菌液浓度10~4时,抑菌浓度为1.5—6.25单位/毫升.突变菌株M-1和M-1-5-5基本保持原出发菌株549的特征,在液体培养时M-1和M-1-5-5的生长速度比549快.试验表明M-1-5-5的抗性基因不在质粒上,连续传代试验说明其抗性具有稳定性.

关键词: 紫外线, 诱变, 联合固氮

Abstract: This paper reports on the two results of Ultra-Violet mutations that oc-curred through radiation treatments for 5 minutes.N ase activity of the selected mutant strain nl-1 is higher than 549, and 1\I-1-5-5 has drug fast of the order 400r-Km. The lowest antibiotic concentration of Km.inhibiting strains 549 and M-1 varies with inoculating bacterial concentrations.It is 1.5-6.25r/ml at 10' of inoculating bacterial concentration. The mutants M-1 and M-1-5-5 retain basically the characteristics of origi-nal strain 549,but they grow ctuiclier than 549 in liquid cultures. The experiments show that the dr ug fast gene of strain M-I-J-J is not in the plasmid and that the drug fast is stable.

Key words: Mutagen, Ultra-violet, Associatve N2-fxng battera

引用本文

周枫, 张启行, 蒋有绎, 张美庆, 刘建玲. 紫外线诱变对联合固氮菌株特性的影响[J]. 华北农学报, 1986, 1(2): 64-69. doi: 10.3321/j.issn:1000-7091.1986.02.010.

Zhou Feng, Zhang Qihang, Jiang Youyi, Zhang Maiqing, Liu Jianling. THE EFFECT OF U V MUTAGEN ON PHYSIOLOGICAL PROPERTIES OF ASSOCIATIVE N2-FIXING BACTERIA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1986, 1(2): 64-69. doi: 10.3321/j.issn:1000-7091.1986.02.010.

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