Abstract: Yak was used as the experimental object to clone the yak CCAAT/Enhancer binding protein alpha(CEBPα) gene, predicted its protein structure and function, isolated yak adipocyte, and detected the expression of CEBPα in different tissues of yak and yak fat expression during cell induction and differentiation. Collected the heart, liver, spleen, kidney, stomach, small intestine, subcutaneous fat, visceral fat and muscle tissue of an adult yak (4 years old), and used PCR technology to obtain the CDS sequence of the CEBPα gene;the method of bioinformatics predicted the sequence homology and protein structure of the gene;Real-time fluorescent quantitative PCR(qRT-PCR) was used to detect the expression of CEBPα in different tissues of yak;the yak preadipocytes were isolated by collagenase digestion, qRT-PCR was used to detect the expression pattern of CEBPα gene at 0, 3, 6, 9 d of induced differentiation;the results of the study showed that the CDS sequence of the yak CEBPα gene was 645 bp in length and encoded 214 amino acids. The yak CEBPα gene had relatively high homology with Bos taurus, with a nucleic acid homology of 99.73% and amino acid homology of 99.19%. The results of constructing a phylogenetic tree showed that the yak was more similar to ordinary cattle and sheep. CEBPα protein was an unstable hydrophilic protein with a transmembrane structure and phosphorylation/dephosphorylation. The secondary structure of the protein was dominated by α helix (27.10%) and random coil (67.29%). The results of qRT-PCR showed that the CEBPα gene was most highly expressed in the lower adipose tissue of the yak, and lowly expressed in the kidney, spleen and visceral adipose tissue. The expression of CEBPα gradually increased during the induction and differentiation of yak adipocytes. In this experiment, we cloned and obtained the CDS region sequence of CEBPα, determined its expression in various tissues of yak and clarified the expression pattern of this gene in the process of induced differentiation of yak adipocytes. Provide basic data to reveal the function of CEBPα gene in yak adipose tissue and adipocyte.

Key words: Yak, CEBPα, Clone, Adipose tissue, Expression pattern

CLC Number: 

• Q78

• S823