Expression and Immunological Characteristics Analysis of the Fragments of the Outer Membrane Protein P5 of Haemophilus parasuis

doi:10.7668/hbnxb.2013.05.009

Abstract

Abstract: This experiment was conducted to express outer membrane protein P 5 of Haemophilus parasuis (HPS) and analysis immunological claracteristics. Three overlapping fragments P5F1,P5F2 and P5F3 of P5 gene were amplified by PCR and cloned into the expression vector pET 32a( +),respectively.It was confirmed that the recombinant plasmids pET32a-P5F1,pET32a-P5F2 and pET32a-P5F3 were constructed correctly by using restric-tion endonuclease digestion and sequencing. The plasmid were transformed into E.coli BL21 ( DE3 ) and the target proteins of 38,32,27 kDa were expressed by induction using IPTG. The immune activity of the proteins was ana-lyzed by Western Blot and ELISA. The results showed that three proteins could react with HPS positive serum. The above results indicated that these recombinant fusion proteins might be useful as an antigen for vaccine and the spe -cific diagnosis antigen for ELISA assay.

Key words: Haemophilus parasuis, Outer membrane protein P5, Analysis of antigenicity

CLC Number:

S852.61

XIE Le-xin, LI Miao, SONG Shuai, YUE Lei, YANG Dong-xia, LI Chun-ling. Expression and Immunological Characteristics Analysis of the Fragments of the Outer Membrane Protein P5 of Haemophilus parasuis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 48-52. doi: 10.7668/hbnxb.2013.05.009.

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