Stability Analysis of Regenerated Plantlets from Dioscorea opposita Germplasm Cryopreserved by Encapsulation-vitrification

doi:10.3969/j.issn.1000-7091.2012.01.044

Abstract

Abstract: In order to verify that cryopreservation technology with encapsulation-vitrification established in an early stage is an effective method of preserving Dioscorea opposita germplasm. From the morphology, physiology and zymogram of isoenzyme, stability analyses of regenerated plantlets from Dioscorea opposita germplasm cryopreserved by encapsulation-vitrification were studied in this paper. The results showed that: compared to plantlets at room temperature, the absolute values of average height, average leaf number, average bud number, average node length, and chlorophyll, soluble protein and soluble sugar contents, superoxide dismutase(SOD) activity of regenerated plantlets from D.opp. cv. Tiegun had all not significantly difference, and the regenerated plantlets could survive after transplanting. In a word, they kept stability of morphology and physiology. Among 4 tested yam cultivars(that is Tiegun, Taigu, Huaiqing and Jiaxiangxichangmao), SOD and protease zymogram, including the band number and strength, there was no difference between regenerated plantlets without cryopreservation and with cryopreservation by encapsulation-vitrification. Cryopreservation by encapsulation-vitrification could keep up stability and be taken as an effective method of Dioscorea opposita germplasm in vitro preservation.

Key words: Dioscorea opposita, Cryopreservation, Encapsulation-vitrification, Genetic stability

CLC Number:

S632.1

ZHAO Xi-ting, WANG Miao, SHAO Huan-juan, LI Xiang, LI Hai-bing, LI Ming-jun. Stability Analysis of Regenerated Plantlets from Dioscorea opposita Germplasm Cryopreserved by Encapsulation-vitrification[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 234-238. doi: 10.3969/j.issn.1000-7091.2012.01.044.

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