Cloning of Homologous Targeting Sequences of Chicory and Construction of Multicistron Chloroplast Site-Specific Integration Expression Vector

doi:10.7668/hbnxb.2010.01.003

Abstract

Abstract: Since chloroplast genomes were highly conservative in evolution,PCR primers were designed basing on the corresponding sequences in the tobacco chloroplast genome.The rps7-rps12-trnV-16S rDNA targeting region(GenBank Accesion Number was GQ199478) was cloned from chicory chloroplast genome,which was used as homologous targeting sequences,and the site-specific integration chicory-specific chloroplast expression vector named pJAG that carries the multicistron expression cassette of prrn-gfp-aadA-psbA-3′ was constructed.The results of restriction analysis were in accord with the desired.This chloroplast expression vector was desirable to be applied in both establishment of chicory chloroplast transformation system and traits improvement of chicory or using chicory chloroplast as bioreactor to produce edible vaccine for animals via plastid genetic transformation.

Key words: Chicory, Chloroplast homologous fragments, Multicistron, Site-specific integration expression vector

CLC Number:

Q789

WANG Yu-hua1,HAN Xiao-ling3,HUANG Cong-lin2,JIA Jing-fen1. Cloning of Homologous Targeting Sequences of Chicory and Construction of Multicistron Chloroplast Site-Specific Integration Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 11-17. doi: 10.7668/hbnxb.2010.01.003.

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http://www.hbnxb.net/EN/Y2010/V25/I1/11

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