Production and Identification of Interspecific Hybrids between Brassica campestris L.ssp.pekinensis and B.nigra

doi:10.7668/hbnxb.2008.04.025

Abstract

Abstract: In order to enrich the germplasm resources of B. campestris, crosses between Brassica. campestris L. pekinensis and B. nigra were made and thirteen hybrids were produced. Three methods including morphology,cytology and SRAP (sequence-related amplified polymorphism) molecular makers were used to verify the hybrids 1 The pollen stain-ability and fertility of the hybrid plants were also analysed.Morphologically the hybrids showed an intermediate phenotype while the dendrogramon SRAP data indicated that the hybrids were homophylic to the female parent. The pollen stainabil-ity of the hybrids was low,among which only one(H4)could produce seeds after backcrossed to the B campestris. Some of the cells in the hybrid H4 had 18 chromosomes while 48 percent of cells contained 26 chromosomes;The other twelve sterility hybrids had 18 or less than 18 chromosomes. Results indicated that the fertility of the hybrids was related to the chromosome number and the increased number of chromosomes could enhance fertility.

Key words: Interspecific hybridization, Brassica. campestris L. ssp. pekinensis, B. nigra, Identification of hybrids

CLC Number:

S634.103.6

WEN Gui-ju, ZHAO Hong, GUO Yang-dong, LIU Fan. Production and Identification of Interspecific Hybrids between Brassica campestris L.ssp.pekinensis and B.nigra[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 105-109. doi: 10.7668/hbnxb.2008.04.025.

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