Abstract:
The auxin of culture medium for inducing callus,bud,root;inoculating method,transplanting were investigated through inoculating 3 kinds of Cyclamen into MS culture medium containing different auxin.The results are as follows.When the explant was the seedling derived from the seed in culture medium,the culture medium for inducing callus was MS+BA 1 5 mg/L+NAA 1 0 mg/L.The culture medium for inducing differentiation bud was MS+BA 1 0 mg/L+NAA 0 5 mg/L.The culture medium for inducing root was MS+NAA 1 0～1 5 mg/L;there was no significant difference among the 3 kinds of Cyclamen.Pollution of endogenetic bacteria was totally eliminated.After 60 d of culture,the amount of callus was 148 0 mm 2,every piece of callus developed 16 2 buds.The root deriving rate was up to 81 9%.This method will certainly become available ways for the developing of Cyclamen in the future.
Key words:
Cyclamen,
Plumule tissue,
Culture Induce
CLC Number:
SU Yu-yuan, WU Yong-zhen, QU Zhan-li, WANG Wen-tao. The Study on Tissue Culture of Cyclamen Plumule[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(S1): 203-205. doi: 10.3321/j.issn:1000-7091.2002.z1.044.