Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2002, Vol. 17 ›› Issue (2): 137-140. doi: 10.3321/j.issn:1000-7091.2002.02.029

Special Issue: Biotechnology；

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Study on an ELISA Kit with Recombinant Antigen for Rapid Diagnosis of Swine Trichinellosis

CHEN Chunhua1, LI Xixian2, YAN Yuhe3, DENG Ruiguang3   

  1. 1. Zhengzhou College of Animal Engineer ing and Veter inary Sciences, Zhengzhou Henan 450008, China;
    21 Sanitation and Antiepidemic Station, Xinhua District, Xinx iang Henan 453000, China;
    3. Institute of Biotechnology , Henan Academy of Agr icultural Sciences, Zhengzhou Henan 450002, China
  • Received:2001-12-02 Published:2002-06-28

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Abstract: An ELISA kit for rapid diagnosis of sw ine t richinosis w as set up. Tw o antigens, gene recombinant antigen(TSRA) and ES antigen, w ere respect ively used in the rapid ELISA to assay the blood samples of 12 pigs infected ex perimentally w ith T richinel la sp iral is , the posit ive rates w ith both ant igens were 100% . The serum and the meat samples of 103 pigs in an endemic regions w ere simultaneously collected and detected w ith TSRA and ES ant igens for a compared test of d-i gestive technique, the diagnost ic agreement rates w ere 95112% and 9613% , respect ively. It is shown that the kit is sensitive, specif ic and reproducible.

Key words: Sw ine trichinellosis, Recombinant ant ig en, ELISA kit

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CHEN Chunhua, LI Xixian, YAN Yuhe, DENG Ruiguang. Study on an ELISA Kit with Recombinant Antigen for Rapid Diagnosis of Swine Trichinellosis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(2): 137-140. doi: 10.3321/j.issn:1000-7091.2002.02.029.

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